2cwq: Difference between revisions

Revision as of 23:14, 3 May 2008

Crystal structure of conserved protein TTHA0727 from Thermus thermophilus HB8

OverviewOverview

TTHA0727 is a conserved hypothetical protein from Thermus thermophilus HB8, with a molecular mass of 12.6 kDa. TTHA0727 belongs to the carboxymuconolactone decarboxylase (CMD) family (Pfam 02627). A sequence comparison with its homologs suggested that TTHA0727 is a distinct protein from alkylhydroperoxidase AhpD and gamma-carboxymuconolactone decarboxylase in the CMD family. Here we report the 1.9 A crystal structure of TTHA0727 (PDB ID: 2CWQ) determined by the multiwavelength anomalous dispersion method. The TTHA0727 monomer structure consists of seven alpha-helices (alpha1-alpha7) and one short 3(10)-helix. The crystal structure and the analytical ultracentrifugation revealed that TTHA0727 forms a hexameric ring structure in solution. The electrostatic potential distribution on the solvent-accessible surface of the TTHA0727 hexamer showed that positively charged regions exist on the side of the ring structure, suggesting that TTHA0727 interacts with some negatively charged molecules. A structural homology search revealed that the structure of three alpha-helices (alpha4-alpha6) is remarkably conserved, suggesting that it is the common structural motif for the CMD family proteins. In addition, the nine residues of the N-terminal tag bound to the cleft region between alpha1 and alpha3 in chains A and B of TTHA0727, implying that this region is the putative binding/active site for some small molecules.

About this StructureAbout this Structure

2CWQ is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the conserved protein TTHA0727 from Thermus thermophilus HB8 at 1.9 A resolution: A CMD family member distinct from carboxymuconolactone decarboxylase (CMD) and AhpD., Ito K, Arai R, Fusatomi E, Kamo-Uchikubo T, Kawaguchi S, Akasaka R, Terada T, Kuramitsu S, Shirouzu M, Yokoyama S, Protein Sci. 2006 May;15(5):1187-92. Epub 2006 Apr 5. PMID:16597838 Page seeded by OCA on Sat May 3 23:13:59 2008

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OCA

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-|GENE= TTHA0727 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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 '''Crystal structure of conserved protein TTHA0727 from Thermus thermophilus HB8'''
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 [[Category: Terada, T.]]
 [[Category: Yokoyama, S.]]
-[[Category: all alpha]]
+[[Category: All alpha]]
-[[Category: conserved hypothetical protein]]
+[[Category: Conserved hypothetical protein]]
-[[Category: national project on protein structural and functional analyse]]
+[[Category: National project on protein structural and functional analyse]]
-[[Category: nppsfa]]
+[[Category: Nppsfa]]
-[[Category: riken structural genomics/proteomics initiative]]
+[[Category: Riken structural genomics/proteomics initiative]]
-[[Category: rsgi]]
+[[Category: Rsgi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:13:59 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:27:22 2008''