2cfp: Difference between revisions

Revision as of 22:01, 3 May 2008

SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH

OverviewOverview

Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.

About this StructureAbout this Structure

2CFP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY., Mirza O, Guan L, Verner G, Iwata S, Kaback HR, EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:16525509 Page seeded by OCA on Sat May 3 22:01:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2cfp.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2cfp |SIZE=350|CAPTION= <scene name='initialview01'>2cfp</scene>, resolution 3.30&Aring;
+The line below this paragraph, containing "STRUCTURE_2cfp", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC2:Hg+Binding+Site+For+Chain+A'>AC2</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
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-|DOMAIN=
+{{STRUCTURE_2cfp|  PDB=2cfp  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfp OCA], [http://www.ebi.ac.uk/pdbsum/2cfp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cfp RCSB]</span>
-}}
 '''SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH'''
@@ Line 30: / Line 27: @@
 [[Category: Mirza, O.]]
 [[Category: Verner, G.]]
-[[Category: formylation]]
+[[Category: Formylation]]
-[[Category: lactose permease]]
+[[Category: Lactose permease]]
-[[Category: lactose/h+ symport]]
+[[Category: Lactose/h+ symport]]
-[[Category: sugar transport]]
+[[Category: Sugar transport]]
-[[Category: transmembrane]]
+[[Category: Transmembrane]]
-[[Category: transport mechanism]]
+[[Category: Transport mechanism]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 22:01:44 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:20:48 2008''