2c8k: Difference between revisions

Revision as of 21:26, 3 May 2008

CRYSTAL STRUCTURE OF (SR) CALCIUM-ATPASE E2(TG) WITH PARTIALLY OCCUPIED AMPPCP SITE

OverviewOverview

We present crystal structures of the calcium-free E2 state of the sarcoplasmic reticulum Ca2+ -ATPase, stabilized by the inhibitor thapsigargin and the ATP analog AMPPCP. The structures allow us to describe the ATP binding site in a modulatory mode uncoupled from the Asp351 phosphorylation site. The Glu439 side chain interacts with AMPPCP via an Mg2+ ion in accordance with previous Fe2+ -cleavage studies implicating this residue in the ATPase cycle and in magnesium binding. Functional data on Ca2+ mediated activation indicate that the crystallized state represents an initial stage of ATP modulated deprotonation of E2, preceding the binding of Ca2+ ions in the membrane from the cytoplasmic side. We propose a mechanism of Ca2+ activation of phosphorylation leading directly from the compact E2-ATP form to the Ca2E1-ATP state. In addition, a role of Glu439 in ATP modulation of other steps of the functional cycle is suggested.

About this StructureAbout this Structure

2C8K is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

ReferenceReference

Modulatory and catalytic modes of ATP binding by the calcium pump., Jensen AM, Sorensen TL, Olesen C, Moller JV, Nissen P, EMBO J. 2006 Jun 7;25(11):2305-14. Epub 2006 May 18. PMID:16710301 Page seeded by OCA on Sat May 3 21:26:49 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:2c8k.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2c8k |SIZE=350|CAPTION= <scene name='initialview01'>2c8k</scene>, resolution 2.80&Aring;
+The line below this paragraph, containing "STRUCTURE_2c8k", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TG1:OCTANOIC+ACID+[3S-[3ALPHA,+3ABETA,+4ALPHA,+6BETA,+6ABETA,+7BETA,+8ALPHA(Z),+9BALPHA]]-6-(ACETYLOXY)-2,3,-3A,4,5,6,6A,7,8,9B-DECAHYDRO-3,3A-DIHYDROXY-3,6,9-TRIMETHYL-8-[(2-METHYL-1-OXO-2-BUTENYL)OXY]-2-OXO-4-(1-OXOBUTOXY)-AZULENO[4,5-B]FURAN-7-YL+ESTER'>TG1</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2c8k|  PDB=2c8k  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8k OCA], [http://www.ebi.ac.uk/pdbsum/2c8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c8k RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF (SR) CALCIUM-ATPASE E2(TG) WITH PARTIALLY OCCUPIED AMPPCP SITE'''
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 [[Category: Olesen, C.]]
 [[Category: Sorensen, T L.]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: ca2+-atpase]]
+[[Category: Ca2+-atpase]]
-[[Category: calcium transport]]
+[[Category: Calcium transport]]
-[[Category: cation pump]]
+[[Category: Cation pump]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: ion transport]]
+[[Category: Ion transport]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
-[[Category: metal-binding]]
+[[Category: Metal-binding]]
-[[Category: modulatory atp]]
+[[Category: Modulatory atp]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: p-type atpase]]
+[[Category: P-type atpase]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:26:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:17:56 2008''