2c5k: Difference between revisions

Revision as of 21:17, 3 May 2008

N-TERMINAL DOMAIN OF TLG1 COMPLEXED WITH N-TERMINUS OF VPS51

OverviewOverview

Membrane fusion in cells involves the interaction of SNARE proteins on apposing membranes. Formation of SNARE complexes is preceded by tethering events, and a number of protein complexes that are thought to mediate this have been identified. The VFT or GARP complex is required for endosome-Golgi traffic in yeast. It consists of four subunits, one of which, Vps51, has been shown to bind specifically to the SNARE Tlg1, which participates in the same fusion event. We have determined the structure of the N-terminal domain of Tlg1 bound to a peptide from the N terminus of Vps51. Binding depends mainly on residues 18-30 of Vps51. These form a short helix which lies in a conserved groove in the three-helix bundle formed by Tlg1. Surprisingly, although both Vps51 and Tlg1 are required for transport to the late Golgi from endosomes, removal of the Tlg1-binding sequences from Vps51 does not block such traffic in vivo. Thus, this particular interaction cannot be crucial to the process of vesicle docking or fusion.

About this StructureAbout this Structure

2C5K is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structural analysis of the interaction between the SNARE Tlg1 and Vps51., Fridmann-Sirkis Y, Kent HM, Lewis MJ, Evans PR, Pelham HR, Traffic. 2006 Feb;7(2):182-90. PMID:16420526 Page seeded by OCA on Sat May 3 21:17:18 2008

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OCA

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 [[Image:2c5k.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2c5k |SIZE=350|CAPTION= <scene name='initialview01'>2c5k</scene>, resolution 2.05&Aring;
+The line below this paragraph, containing "STRUCTURE_2c5k", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+T'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2c5k|  PDB=2c5k  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c5k OCA], [http://www.ebi.ac.uk/pdbsum/2c5k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c5k RCSB]</span>
-}}
 '''N-TERMINAL DOMAIN OF TLG1 COMPLEXED WITH N-TERMINUS OF VPS51'''
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 [[Category: Lewis, M J.]]
 [[Category: Pelham, H R.B.]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: protein transport]]
+[[Category: Protein transport]]
-[[Category: snare]]
+[[Category: Snare]]
-[[Category: vft complex]]
+[[Category: Vft complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:17:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:16:42 2008''