2c4b: Difference between revisions

Revision as of 21:13, 3 May 2008

INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A

OverviewOverview

We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.

About this StructureAbout this Structure

2C4B is a Single protein structure of sequence from Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium. Full crystallographic information is available from OCA.

ReferenceReference

Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652 Page seeded by OCA on Sat May 3 21:13:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2c4b.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2c4b |SIZE=350|CAPTION= <scene name='initialview01'>2c4b</scene>, resolution 1.30&Aring;
+The line below this paragraph, containing "STRUCTURE_2c4b", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2c4b|  PDB=2c4b  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c4b OCA], [http://www.ebi.ac.uk/pdbsum/2c4b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c4b RCSB]</span>
-}}
 '''INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A'''
@@ Line 24: / Line 21: @@
 Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16337652 16337652]
 [[Category: Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]]
-[[Category: Ribonuclease T(1)]]
 [[Category: Single protein]]
 [[Category: Heinz, D W.]]
@@ Line 31: / Line 27: @@
 [[Category: Schmoldt, H U.]]
 [[Category: Wentzel, A.]]
-[[Category: endonuclease]]
+[[Category: Endonuclease]]
-[[Category: fusion protein]]
+[[Category: Fusion protein]]
-[[Category: hybrid microprotein]]
+[[Category: Hybrid microprotein]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: nuclease]]
+[[Category: Nuclease]]
-[[Category: protease inhibitor]]
+[[Category: Protease inhibitor]]
-[[Category: ribonuclease]]
+[[Category: Ribonuclease]]
-[[Category: serine protease inhibitor]]
+[[Category: Serine protease inhibitor]]
-[[Category: squash inhibitor]]
+[[Category: Squash inhibitor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:13:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:16:04 2008''