2by5: Difference between revisions

Revision as of 20:57, 3 May 2008

IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION

OverviewOverview

This paper focuses on the radiation-damage effects when applying the same total X-ray dose to protein crystals at different dose rates. These experiments have been performed on both a selenomethionated protein and on bovine trypsin using dose rates that span nearly two orders of magnitude. The results show no clear dose-rate effect on the global indicators of radiation damage, but a small measurable dose-rate effect could be found when studying specific radiation damage. It is hypothesized that this observed dose-rate effect relates to differences in the steady-state free-radical concentration.

About this StructureAbout this Structure

2BY5 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442 Page seeded by OCA on Sat May 3 20:57:27 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2by5.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2by5 |SIZE=350|CAPTION= <scene name='initialview01'>2by5</scene>, resolution 1.30&Aring;
+The line below this paragraph, containing "STRUCTURE_2by5", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+X'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2by5|  PDB=2by5  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2by5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2by5 OCA], [http://www.ebi.ac.uk/pdbsum/2by5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2by5 RCSB]</span>
-}}
 '''IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION'''
@@ Line 30: / Line 27: @@
 [[Category: Ravelli, R B.G.]]
 [[Category: Timmins, J.]]
-[[Category: data collection,radiation damage,dose-rate,synchrotron radiation,hydrolase]]
+[[Category: Data collection,radiation damage,dose-rate,synchrotron radiation,hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:57:27 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:13:32 2008''