2bnx: Difference between revisions
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'''CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1''' | '''CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1''' | ||
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[[Category: Rosen, M.]] | [[Category: Rosen, M.]] | ||
[[Category: Tomchick, D R.]] | [[Category: Tomchick, D R.]] | ||
[[Category: | [[Category: Actin]] | ||
[[Category: | [[Category: Autoinhibition]] | ||
[[Category: | [[Category: Cytoskeleton]] | ||
[[Category: | [[Category: Nucleation]] | ||
[[Category: | [[Category: Structural protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:32:56 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 20:32, 3 May 2008
CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1
OverviewOverview
Diaphanous-related formins (DRFs) regulate dynamics of unbranched actin filaments during cell contraction and cytokinesis. DRFs are autoinhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-terminal regulatory element. Autoinhibition is relieved through binding of the GTPase RhoA to the N-terminal element. We report the crystal structure of the dimeric regulatory domain of the DRF, mDia1. Dimerization is mediated by an intertwined six-helix bundle, from which extend two Diaphanous inhibitory domains (DIDs) composed of five armadillo repeats. NMR and biochemical mapping indicate the RhoA and DAD binding sites on the DID partially overlap, explaining activation of mDia1 by the GTPase. RhoA binding also requires an additional structurally independent segment adjacent to the DID. This regulatory construction, involving a GTPase binding site spanning a flexibly tethered arm and the inhibitory module, is observed in many autoinhibited effectors of Ras superfamily GTPases, suggesting evolutionary pressure for this design.
About this StructureAbout this Structure
2BNX is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of Rho GTPase-mediated activation of the formin mDia1., Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK, Mol Cell. 2005 Apr 29;18(3):273-81. PMID:15866170 Page seeded by OCA on Sat May 3 20:32:56 2008