2bku: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
No edit summary
No edit summary
Line 1: Line 1:
[[Image:2bku.gif|left|200px]]
[[Image:2bku.gif|left|200px]]


{{Structure
<!--
|PDB= 2bku |SIZE=350|CAPTION= <scene name='initialview01'>2bku</scene>, resolution 2.70&Aring;
The line below this paragraph, containing "STRUCTURE_2bku", creates the "Structure Box" on the page.
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
|LIGAND= <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY=  
or leave the SCENE parameter empty for the default display.
|GENE=  
-->
|DOMAIN=
{{STRUCTURE_2bku| PDB=2bku  | SCENE= }}  
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bku OCA], [http://www.ebi.ac.uk/pdbsum/2bku PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bku RCSB]</span>
}}


'''KAP95P:RANGTP COMPLEX'''
'''KAP95P:RANGTP COMPLEX'''
Line 31: Line 28:
[[Category: Matsuura, Y.]]
[[Category: Matsuura, Y.]]
[[Category: Stewart, M.]]
[[Category: Stewart, M.]]
[[Category: gtp binding]]
[[Category: Gtp binding]]
[[Category: importin-beta]]
[[Category: Importin-beta]]
[[Category: nuclear transport]]
[[Category: Nuclear transport]]
[[Category: protein transport]]
[[Category: Protein transport]]
[[Category: rangtp]]
[[Category: Rangtp]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:25:37 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:55 2008''

Revision as of 20:25, 3 May 2008

File:2bku.gif

Template:STRUCTURE 2bku

KAP95P:RANGTP COMPLEX


OverviewOverview

Nuclear protein import is mediated mainly by the transport factor importin-beta that binds cytoplasmic cargo, most often via the importin-alpha adaptor, and then transports it through nuclear pore complexes. This active transport is driven by disassembly of the import complex by nuclear RanGTP. The switch I and II loops of Ran change conformation with nucleotide state, and regulate its interactions with nuclear trafficking components. Importin-beta consists of 19 HEAT repeats that are based on a pair of antiparallel alpha-helices (referred to as the A- and B-helices). The HEAT repeats stack to yield two C-shaped arches, linked together to form a helicoidal molecule that has considerable conformational flexibility. Here we present the structure of full-length yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides a basis for understanding the crucial cargo-release step of nuclear import. We identify a key interaction site where the RanGTP switch I loop binds to the carboxy-terminal arch of Kap95p. This interaction produces a change in helicoidal pitch that locks Kap95p in a conformation that cannot bind importin-alpha or cargo. We suggest an allosteric mechanism for nuclear import complex disassembly by RanGTP.

About this StructureAbout this Structure

2BKU is a Protein complex structure of sequences from Canis lupus familiaris and Saccharomyces cerevisiae. The following page contains interesting information on the relation of 2BKU with [Importins]. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for nuclear import complex dissociation by RanGTP., Lee SJ, Matsuura Y, Liu SM, Stewart M, Nature. 2005 Jun 2;435(7042):693-6. Epub 2005 May 1. PMID:15864302 Page seeded by OCA on Sat May 3 20:25:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA