4b5n: Difference between revisions
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==Crystal structure of oxidized Shewanella Yellow Enzyme 4 (SYE4)== | ==Crystal structure of oxidized Shewanella Yellow Enzyme 4 (SYE4)== | ||
<StructureSection load='4b5n' size='340' side='right' caption='[[4b5n]], [[Resolution|resolution]] 1.10Å' scene=''> | <StructureSection load='4b5n' size='340' side='right'caption='[[4b5n]], [[Resolution|resolution]] 1.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4b5n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sheon Sheon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B5N FirstGlance]. <br> | <table><tr><td colspan='2'>[[4b5n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sheon Sheon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B5N FirstGlance]. <br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: NADPH dehydrogenase]] | [[Category: NADPH dehydrogenase]] | ||
[[Category: Sheon]] | [[Category: Sheon]] | ||
Revision as of 19:50, 28 August 2019
Crystal structure of oxidized Shewanella Yellow Enzyme 4 (SYE4)Crystal structure of oxidized Shewanella Yellow Enzyme 4 (SYE4)
Structural highlights
Publication Abstract from PubMedShewanella oneidensis, a Gram-negative gamma-proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a series of high-resolution crystal structures for SYE4 in the oxidized and reduced states, and in complex with phenolic ligands and the nitro-aromatic explosive picric acid. The structures unmask new features, including the identification of a binding platform for long-chain hydrophobic molecules. Furthermore, we present the first structural observation of a hydride-Meisenheimer complex of picric acid with a flavoenzyme. Overall, our study exposes the binding promiscuity of SYE4 toward a variety of electrophilic substrates and is consistent with a general detoxification function for SYE4. Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands.,Elegheert J, Brige A, Van Beeumen J, Savvides SN FEBS Lett. 2017 Sep 4. doi: 10.1002/1873-3468.12833. PMID:28869767[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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