2bcs: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2bcs.gif|left|200px]] | [[Image:2bcs.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2bcs", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_2bcs| PDB=2bcs | SCENE= }} | |||
}} | |||
'''DNA polymerase lambda in complex with a DNA duplex containing an unpaired Dcmp''' | '''DNA polymerase lambda in complex with a DNA duplex containing an unpaired Dcmp''' | ||
| Line 30: | Line 27: | ||
[[Category: Kunkel, T A.]] | [[Category: Kunkel, T A.]] | ||
[[Category: Pedersen, L C.]] | [[Category: Pedersen, L C.]] | ||
[[Category: | [[Category: Deletion]] | ||
[[Category: | [[Category: Extrahelical]] | ||
[[Category: | [[Category: Misalignment]] | ||
[[Category: | [[Category: Mutagenesis]] | ||
[[Category: | [[Category: Mutation]] | ||
[[Category: | [[Category: Slippage]] | ||
[[Category: | [[Category: Streisinger]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:07:11 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 20:07, 3 May 2008
DNA polymerase lambda in complex with a DNA duplex containing an unpaired Dcmp
OverviewOverview
Insertions and deletions in coding sequences can alter the reading frame of genes and have profound biological consequences. In 1966, Streisinger proposed that these mutations result from strand slippage, which in repetitive sequences generates misaligned intermediates stabilized by correct base pairing that support polymerization. We report here crystal structures of human DNA polymerase lambda, which frequently generates deletion mutations, bound to such intermediates. Each contains an extrahelical template nucleotide upstream of the active site. Surprisingly, the extra nucleotide, even when combined with an adjacent mismatch, does not perturb polymerase active site geometry, which is indistinguishable from that for correctly aligned strands. These structures reveal how pol lambda can polymerize on substrates with minimal homology during repair of double-strand breaks and represent strand-slippage intermediates consistent with Streisinger's classical hypothesis. They are thus relevant to the origin of single-base deletions, a class of mutations that can confer strong biological phenotypes.
About this StructureAbout this Structure
2BCS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of strand misalignment during DNA synthesis by a human DNA polymerase., Garcia-Diaz M, Bebenek K, Krahn JM, Pedersen LC, Kunkel TA, Cell. 2006 Jan 27;124(2):331-42. PMID:16439207 Page seeded by OCA on Sat May 3 20:07:11 2008