2bc3: Difference between revisions

Revision as of 20:05, 3 May 2008

T7-tagged full-length streptavidin

OverviewOverview

The structure of a full-length streptavidin has been determined at 1.7 A resolution and shows that the 20 residue extension at the C terminus forms a well-ordered polypeptide loop on the surface of the tetramer. Residues 150-153 of the extension are bound to the ligand-binding site, possibly competing with exogenous ligands. The binding mode of these residues is compared with that of biotin and peptidic ligands. The observed structure helps to rationalize the observations that full-length mature streptavidin binds biotinylated macromolecules with reduced affinity.

About this StructureAbout this Structure

2BC3 is a Single protein structure of sequence from Streptomyces avidinii. Full crystallographic information is available from OCA.

ReferenceReference

Crystallographic analysis of a full-length streptavidin with its C-terminal polypeptide bound in the biotin binding site., Le Trong I, Humbert N, Ward TR, Stenkamp RE, J Mol Biol. 2006 Feb 24;356(3):738-45. Epub 2005 Dec 15. PMID:16384581 Page seeded by OCA on Sat May 3 20:05:53 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2bc3.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_2bc3", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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-|GENE=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bc3 OCA], [http://www.ebi.ac.uk/pdbsum/2bc3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bc3 RCSB]</span>
-}}
 '''T7-tagged full-length streptavidin'''
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 [[Category: Trong, I Le.]]
 [[Category: Ward, T R.]]
-[[Category: streptavidin]]
+[[Category: Streptavidin]]
-[[Category: t7 tag]]
+[[Category: T7 tag]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:05:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:04:10 2008''