5fff: Difference between revisions
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==Noroxomaritidine/Norcraugsodine Reductase in complex with NADP+ and piperonal== | ==Noroxomaritidine/Norcraugsodine Reductase in complex with NADP+ and piperonal== | ||
<StructureSection load='5fff' size='340' side='right' caption='[[5fff]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='5fff' size='340' side='right'caption='[[5fff]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5fff]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Daffodil Daffodil]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FFF FirstGlance]. <br> | <table><tr><td colspan='2'>[[5fff]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Daffodil Daffodil]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FFF FirstGlance]. <br> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Daffodil]] | [[Category: Daffodil]] | ||
[[Category: Large Structures]] | |||
[[Category: Holland, C K]] | [[Category: Holland, C K]] | ||
[[Category: Jez, J M]] | [[Category: Jez, J M]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Short-chain dehydrogenase/reductase alkaloid biosynthesis]] | [[Category: Short-chain dehydrogenase/reductase alkaloid biosynthesis]] | ||
Revision as of 14:38, 25 December 2019
Noroxomaritidine/Norcraugsodine Reductase in complex with NADP+ and piperonalNoroxomaritidine/Norcraugsodine Reductase in complex with NADP+ and piperonal
Structural highlights
Publication Abstract from PubMedAmaryllidaceae alkaloids are a large group of plant natural products with over 300 documented structures and diverse biological activities. Several groups of Amaryllidaceae alkaloids including the haemanthamine and crinine type alkaloids show promise as anticancer agents. Two reduction reactions are required for the production of these compounds: the reduction of norcraugsodine to norbelladine and the reduction of noroxomaritidine to normaritidine, with the enantiomer of noroxomaritidine dictating whether the derivatives will be the crinine type or haemanthamine type. It is also possible for the carbon-carbon double bond of noroxomaritidine to be reduced, forming the precursor for maritinamine or elwesine depending on the enantiomer reduced to an oxomaritinamine product. In this study, a short-chain alcohol dehydrogenase/reductase that co-expresses with the previously discovered norbelladine 4'-O-methyltransferase from Narcissus sp. and Galanthus spp. was cloned and expressed in E. coli. Biochemical analyses and x-ray crystallography indicates that this protein functions as a noroxomaritidine reductase that forms oxomaritinamine from noroxomaritidine through a carbon-carbon double bond reduction. The enzyme also reduces norcraugsodine to norbelladine with a 400-fold lower specific activity. These studies identify a missing step in the biosynthesis of this pharmacologically important class of plant natural products. Identification of a Noroxomaritidine Reductase with Amaryllidaceae Alkaloid Biosynthesis Related Activities.,Kilgore MB, Holland CK, Jez JM, Kutchan TM J Biol Chem. 2016 Jun 1. pii: jbc.M116.717827. PMID:27252378[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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