2aq4: Difference between revisions

Revision as of 19:20, 3 May 2008

Ternary complex of the catalytic core of REV1 with DNA and dCTP.

OverviewOverview

The Rev1 DNA polymerase is highly specialized for the incorporation of C opposite template G. We present here the crystal structure of yeast Rev1 bound to template G and incoming 2'-deoxycytidine 5'-triphosphate (dCTP), which reveals that the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base. Template G and incoming dCTP do not pair with each other. Instead, the template G is evicted from the DNA helix, and it makes optimal hydrogen bonds with a segment of Rev1. Also, unlike other DNA polymerases, incoming dCTP pairs with an arginine rather than the templating base, which ensures the incorporation of dCTP over other incoming nucleotides. This mechanism provides an elegant means for promoting proficient and error-free synthesis through N2-adducted guanines that obstruct replication.

About this StructureAbout this Structure

2AQ4 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Rev1 employs a novel mechanism of DNA synthesis using a protein template., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Science. 2005 Sep 30;309(5744):2219-22. PMID:16195463 Page seeded by OCA on Sat May 3 19:20:02 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2aq4.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2aq4 |SIZE=350|CAPTION= <scene name='initialview01'>2aq4</scene>, resolution 2.32&Aring;
+The line below this paragraph, containing "STRUCTURE_2aq4", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DCP:2&#39;-DEOXYCYTIDINE-5&#39;-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DOC:2&#39;,3&#39;-DIDEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= REV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_2aq4|  PDB=2aq4  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aq4 OCA], [http://www.ebi.ac.uk/pdbsum/2aq4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aq4 RCSB]</span>
-}}
 '''Ternary complex of the catalytic core of REV1 with DNA and dCTP.'''
@@ Line 30: / Line 27: @@
 [[Category: Prakash, L.]]
 [[Category: Prakash, S.]]
-[[Category: g-loop]]
+[[Category: G-loop]]
-[[Category: n-digit]]
+[[Category: N-digit]]
-[[Category: pad]]
+[[Category: Pad]]
-[[Category: polymerase]]
+[[Category: Polymerase]]
-[[Category: rev1]]
+[[Category: Rev1]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:20:02 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:55:45 2008''