2ajt: Difference between revisions

Revision as of 19:08, 3 May 2008

Crystal structure of L-Arabinose Isomerase from E.coli

OverviewOverview

Escherichia coli L-arabinose isomerase (ECAI; EC 5.3.1.4) catalyzes the isomerization of L-arabinose to L-ribulose in vivo. This enzyme is also of commercial interest as it catalyzes the conversion of D-galactose to D-tagatose in vitro. The crystal structure of ECAI was solved and refined at 2.6 A resolution. The subunit structure of ECAI is organised into three domains: an N-terminal, a central and a C-terminal domain. It forms a crystallographic trimeric architecture in the asymmetric unit. Packing within the crystal suggests the idea that ECAI can form a hexameric assembly. Previous electron microscopic and biochemical studies supports that ECAI is hexameric in solution. A comparison with other known structures reveals that ECAI adopts a protein fold most similar to E. coli fucose isomerase (ECFI) despite very low sequence identity 9.7%. The structural similarity between ECAI and ECFI with regard to number of domains, overall fold, biological assembly, and active site architecture strongly suggests that the enzymes have functional similarities. Further, the crystal structure of ECAI forms a basis for identifying molecular determinants responsible for isomerization of arabinose to ribulose in vivo and galactose to tagatose in vitro.

About this StructureAbout this Structure

2AJT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production., Manjasetty BA, Chance MR, J Mol Biol. 2006 Jul 7;360(2):297-309. Epub 2006 May 5. PMID:16756997 Page seeded by OCA on Sat May 3 19:08:30 2008

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OCA

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 [[Image:2ajt.gif|left|200px]]
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 '''Crystal structure of L-Arabinose Isomerase from E.coli'''
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 [[Category: Manjasetty, B A.]]
 [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
-[[Category: arabinose catabolism]]
+[[Category: Arabinose catabolism]]
-[[Category: carbohydrate metabolism]]
+[[Category: Carbohydrate metabolism]]
-[[Category: isomerase]]
+[[Category: Isomerase]]
-[[Category: new york structural genomix research consortium]]
+[[Category: New york structural genomix research consortium]]
-[[Category: nysgxrc]]
+[[Category: Nysgxrc]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:08:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:53:48 2008''