2afi: Difference between revisions

Revision as of 18:59, 3 May 2008

Crystal Structure of MgADP bound Av2-Av1 Complex

OverviewOverview

Adenosine triphosphate (ATP) hydrolysis in the nitrogenase complex controls the cycle of association and dissociation between the electron donor adenosine triphosphatase (ATPase) (Fe-protein) and its target catalytic protein (MoFe-protein), driving the reduction of dinitrogen into ammonia. Crystal structures in different nucleotide states have been determined that identify conformational changes in the nitrogenase complex during ATP turnover. These structures reveal distinct and mutually exclusive interaction sites on the MoFe-protein surface that are selectively populated, depending on the Fe-protein nucleotide state. A consequence of these different docking geometries is that the distance between redox cofactors, a critical determinant of the intermolecular electron transfer rate, is coupled to the nucleotide state. More generally, stabilization of distinct docking geometries by different nucleotide states, as seen for nitrogenase, could enable nucleotide hydrolysis to drive the relative motion of protein partners in molecular motors and other systems.

About this StructureAbout this Structure

2AFI is a Protein complex structure of sequences from Azotobacter vinelandii. Full crystallographic information is available from OCA.

ReferenceReference

Nitrogenase complexes: multiple docking sites for a nucleotide switch protein., Tezcan FA, Kaiser JT, Mustafi D, Walton MY, Howard JB, Rees DC, Science. 2005 Aug 26;309(5739):1377-80. PMID:16123301 Page seeded by OCA on Sat May 3 18:59:26 2008

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OCA

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 [[Image:2afi.gif|left|200px]]
- {{Structure
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2afi|  PDB=2afi  |  SCENE=  }}
-|RELATEDENTRY=[[2afh|2AFH]], [[2afk|2AFK]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2afi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afi OCA], [http://www.ebi.ac.uk/pdbsum/2afi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2afi RCSB]</span>
-}}
 '''Crystal Structure of MgADP bound Av2-Av1 Complex'''
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 [[Category: Tezcan, F A.]]
 [[Category: Walton, M Y.]]
-[[Category: iron-sulfur]]
+[[Category: Iron-sulfur]]
-[[Category: metal-binding]]
+[[Category: Metal-binding]]
-[[Category: molybdenum]]
+[[Category: Molybdenum]]
-[[Category: nitrogen fixation]]
+[[Category: Nitrogen fixation]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:59:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:51:59 2008''