2adw: Difference between revisions

Revision as of 18:55, 3 May 2008

Crystal strucutre of Echinomycin-(ACGTACGT)2 solved by SAD

OverviewOverview

A new crystal form was obtained for the complex between (ACGTACGT)2 and echinomycin and X-ray data were collected to 1.6 A. The structure was phased by the SAD method based on a single unexpected anomalous scatterer that could be identified as a mixture of nickel and zinc by measurements of the anomalous scattering at different wavelengths. This cation is coordinated by two guanines from two different duplexes and four water molecules. The structure resembles previously reported crystal structures of DNA-echinomycin complexes, except that three of the eight base pairs flanking the echinomycin bisintercalator sites have the Watson-Crick rather than the Hoogsteen configuration. Hoogsteen binding was found for the corresponding base pairs of the crystallographically independent duplex, indicating that the two configurations are very close in energy.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Serendipitous SAD phasing of an echinomycin-(ACGTACGT)2 bisintercalation complex., Cuesta-Seijo JA, Weiss MS, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):417-24. Epub 2006, Mar 18. PMID:16552143 Page seeded by OCA on Sat May 3 18:55:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2adw.gif|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1pfe|1PFE]], [[1xvk|1XVK]], [[1xvn|1XVN]], [[1xvr|1XVR]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adw OCA], [http://www.ebi.ac.uk/pdbsum/2adw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2adw RCSB]</span>
-}}
 '''Crystal strucutre of Echinomycin-(ACGTACGT)2 solved by SAD'''
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 ==About this Structure==
-ADW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADW OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADW OCA].
 ==Reference==
 Serendipitous SAD phasing of an echinomycin-(ACGTACGT)2 bisintercalation complex., Cuesta-Seijo JA, Weiss MS, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):417-24. Epub 2006, Mar 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16552143 16552143]
-[[Category: Protein complex]]
 [[Category: Cuesta-Seijo, J A.]]
 [[Category: Sheldrick, G M.]]
-[[Category: bisintercalator]]
+[[Category: Bisintercalator]]
-[[Category: hoogsteen/watson-crick]]
+[[Category: Hoogsteen/watson-crick]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:55:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:51:16 2008''