2ab5: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2ab5.gif|left|200px]] | [[Image:2ab5.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2ab5", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_2ab5| PDB=2ab5 | SCENE= }} | |||
| | |||
| | |||
}} | |||
'''bI3 LAGLIDADG Maturase''' | '''bI3 LAGLIDADG Maturase''' | ||
| Line 32: | Line 29: | ||
[[Category: Longo, A.]] | [[Category: Longo, A.]] | ||
[[Category: Weeks, K M.]] | [[Category: Weeks, K M.]] | ||
[[Category: | [[Category: Group i intron splicing]] | ||
[[Category: | [[Category: Laglidadg endonuclease]] | ||
[[Category: | [[Category: Maturase]] | ||
[[Category: | [[Category: Rna binding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:50:21 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 18:50, 3 May 2008
bI3 LAGLIDADG Maturase
OverviewOverview
LAGLIDADG endonucleases bind across adjacent major grooves via a saddle-shaped surface and catalyze DNA cleavage. Some LAGLIDADG proteins, called maturases, facilitate splicing by group I introns, raising the issue of how a DNA-binding protein and an RNA have evolved to function together. In this report, crystallographic analysis shows that the global architecture of the bI3 maturase is unchanged from its DNA-binding homologs; in contrast, the endonuclease active site, dispensable for splicing facilitation, is efficiently compromised by a lysine residue replacing essential catalytic groups. Biochemical experiments show that the maturase binds a peripheral RNA domain 50 A from the splicing active site, exemplifying long-distance structural communication in a ribonucleoprotein complex. The bI3 maturase nucleic acid recognition saddle interacts at the RNA minor groove; thus, evolution from DNA to RNA function has been mediated by a switch from major to minor groove interaction.
About this StructureAbout this Structure
2AB5 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Evolution from DNA to RNA recognition by the bI3 LAGLIDADG maturase., Longo A, Leonard CW, Bassi GS, Berndt D, Krahn JM, Hall TM, Weeks KM, Nat Struct Mol Biol. 2005 Sep;12(9):779-87. Epub 2005 Aug 21. PMID:16116439 Page seeded by OCA on Sat May 3 18:50:21 2008