1ogv: Difference between revisions
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[[Image:1ogv.gif|left|200px]]<br /> | [[Image:1ogv.gif|left|200px]]<br /><applet load="1ogv" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1ogv" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1ogv, resolution 2.35Å" /> | caption="1ogv, resolution 2.35Å" /> | ||
'''LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES'''<br /> | '''LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1OGV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE2, CL, BCL, BPH, U10 and CDL as [http://en.wikipedia.org/wiki/ligands ligands]. | 1OGV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE2, CL, BCL, BPH, U10 and CDL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain M'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:42:48 2007'' | ||
Revision as of 18:33, 18 December 2007
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LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES
OverviewOverview
Well-ordered crystals of the bacterial photosynthetic reaction centre from, Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we, report the type I crystal packing that results from this crystallisation, medium, for which 3D crystals grow as stacked 2D crystals, and the, reaction centre X-ray structure is refined to 2.35A resolution. In this, crystal form, the location of the membrane bilayer could be assigned with, confidence. A cardiolipin-binding site is found at the protein-protein, interface within the membrane-spanning region, shedding light on the, formation of crystal contacts within the membrane. A chloride-binding site, was identified in the membrane-spanning region, which suggests a putative, site for interaction with the light-harvesting complex I, the cytochrome, bc(1) complex or PufX. Comparisons with the X-ray structures of this, reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich, environment.
About this StructureAbout this Structure
1OGV is a Protein complex structure of sequences from Rhodobacter sphaeroides with FE2, CL, BCL, BPH, U10 and CDL as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution., Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R, J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:12899837
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