2a87: Difference between revisions

Revision as of 18:43, 3 May 2008

Crystal Structure of M. tuberculosis Thioredoxin reductase

OverviewOverview

The thioredoxin system exists ubiquitously and participates in essential antioxidant and redox-regulation processes via a pair of conserved cysteine residues. In Mycobacterium tuberculosis, which lacks a genuine glutathione system, the thioredoxin system provides reducing equivalents inside the cell. The three-dimensional structure of thioredoxin reductase from M. tuberculosis has been determined at 3 A resolution. TLS refinement reveals a large libration axis, showing that NADPH-binding domain has large anisotropic disorder. The relative rotation of the NADPH domain with respect to the FAD domain is necessary for the thioredoxin reduction cycle, as it brings the spatially distant reacting sites close together. Normal-mode analysis carried out based on the elastic network model shows that the motion required to bring about the functional conformational change can be accounted for by motion along one single mode. TLS refinement and normal-mode analysis thus enhance our understanding of the associated conformational changes.

About this StructureAbout this Structure

2A87 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Conformational flexibility of Mycobacterium tuberculosis thioredoxin reductase: crystal structure and normal-mode analysis., Akif M, Suhre K, Verma C, Mande SC, Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1603-11. Epub 2005, Nov 19. PMID:16301794 Page seeded by OCA on Sat May 3 18:43:57 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2a87.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2a87 |SIZE=350|CAPTION= <scene name='initialview01'>2a87</scene>, resolution 3.00&Aring;
+The line below this paragraph, containing "STRUCTURE_2a87", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= trxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00070 Pyr_redox], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0492 TrxB]</span>
+{{STRUCTURE_2a87|  PDB=2a87  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a87 OCA], [http://www.ebi.ac.uk/pdbsum/2a87 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a87 RCSB]</span>
-}}
 '''Crystal Structure of M. tuberculosis Thioredoxin reductase'''
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 [[Category: TBSGC, TB Structural Genomics Consortium.]]
 [[Category: Verma, C.]]
-[[Category: fad]]
+[[Category: Fad]]
-[[Category: nap]]
+[[Category: Nap]]
-[[Category: nma]]
+[[Category: Nma]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: tb structural genomics consortium]]
+[[Category: Tb structural genomics consortium]]
-[[Category: tbsgc]]
+[[Category: Tbsgc]]
-[[Category: thioredoxin reductase]]
+[[Category: Thioredoxin reductase]]
-[[Category: tl]]
+[[Category: Tl]]
-[[Category: trxr]]
+[[Category: Trxr]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:43:57 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:49:12 2008''