2a7b: Difference between revisions

Revision as of 18:42, 3 May 2008

On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength

OverviewOverview

Complete and highly redundant data sets were collected at different wavelengths between 0.80 and 2.65 A for a total of ten different protein and DNA model systems. The magnitude of the anomalous signal-to-noise ratio as assessed by the quotient R(anom)/R(r.i.m.) was found to be influenced by the data-collection wavelength and the nature of the anomalously scattering substructure. By utilizing simple empirical correlations, for instance between the estimated deltaF/F and the expected R(anom) or the data-collection wavelength and the expected R(r.i.m.), the wavelength at which the highest anomalous signal-to-noise ratio can be expected could be estimated even before the experiment. Almost independent of the nature of the anomalously scattering substructure and provided that no elemental X-ray absorption edge is nearby, this optimal wavelength is 2.1 A.

About this StructureAbout this Structure

2A7B is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength., Mueller-Dieckmann C, Panjikar S, Tucker PA, Weiss MS, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1263-72. Epub 2005, Aug 16. PMID:16131760 Page seeded by OCA on Sat May 3 18:42:01 2008

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OCA

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 [[Image:2a7b.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_2a7b", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=XE:XENON'>XE</scene>
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-|GENE= Ap1g1, Adtg, Clapg1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
+{{STRUCTURE_2a7b|  PDB=2a7b  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7b OCA], [http://www.ebi.ac.uk/pdbsum/2a7b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a7b RCSB]</span>
-}}
 '''On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength'''
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 [[Category: Tucker, P A.]]
 [[Category: Weiss, M S.]]
-[[Category: protein transport]]
+[[Category: Protein transport]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:42:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:48 2008''