2a6m: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2a6m.gif|left|200px]] | [[Image:2a6m.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2a6m", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
| | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| | or leave the SCENE parameter empty for the default display. | ||
--> | |||
{{STRUCTURE_2a6m| PDB=2a6m | SCENE= }} | |||
}} | |||
'''Crystal Structure of the ISHp608 Transposase''' | '''Crystal Structure of the ISHp608 Transposase''' | ||
| Line 32: | Line 29: | ||
[[Category: Ronning, D R.]] | [[Category: Ronning, D R.]] | ||
[[Category: Ton-Hoang, B.]] | [[Category: Ton-Hoang, B.]] | ||
[[Category: | [[Category: Rna recognition motif]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:40:21 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 18:40, 3 May 2008
Crystal Structure of the ISHp608 Transposase
OverviewOverview
Many bacteria harbor simple transposable elements termed insertion sequences (IS). In Helicobacter pylori, the chimeric IS605 family elements are particularly interesting due to their proximity to genes encoding gastric epithelial invasion factors. Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases. We have solved the crystal structure of full-length transposase (TnpA) of a representative member, ISHp608. Structurally, TnpA does not resemble any characterized transposase; rather, it is related to rolling circle replication (RCR) proteins. Consistent with RCR, Mg2+ and a conserved tyrosine, Tyr127, are essential for DNA nicking and the formation of a covalent intermediate between TnpA and DNA. TnpA is dimeric, contains two shared active sites, and binds two DNA stem loops representing the conserved inverted repeats near each end of ISHp608. The cocrystal structure with stem-loop DNA illustrates how this family of transposases specifically recognizes and pairs ends, necessary steps during transposition.
About this StructureAbout this Structure
2A6M is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.
ReferenceReference
Active site sharing and subterminal hairpin recognition in a new class of DNA transposases., Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F, Mol Cell. 2005 Oct 7;20(1):143-54. PMID:16209952 Page seeded by OCA on Sat May 3 18:40:21 2008