5sv1: Difference between revisions
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==Structure of the ExbB/ExbD complex from E. coli at pH 4.5== | ==Structure of the ExbB/ExbD complex from E. coli at pH 4.5== | ||
<StructureSection load='5sv1' size='340' side='right' caption='[[5sv1]], [[Resolution|resolution]] 3.50Å' scene=''> | <StructureSection load='5sv1' size='340' side='right'caption='[[5sv1]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5sv1]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecod1 Ecod1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SV1 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5sv1]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecod1 Ecod1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SV1 FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5sv1" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5sv1" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[ExbB|ExbB]] | |||
*[[ExbD|ExbD]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Ecod1]] | [[Category: Ecod1]] | ||
[[Category: Large Structures]] | |||
[[Category: Botos, I]] | [[Category: Botos, I]] | ||
[[Category: Buchanan, S K]] | [[Category: Buchanan, S K]] | ||
Revision as of 19:51, 11 December 2019
Structure of the ExbB/ExbD complex from E. coli at pH 4.5Structure of the ExbB/ExbD complex from E. coli at pH 4.5
Structural highlights
Function[EXBB_ECO57] Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Protects ExbD from proteolytic degradation and functionally stabilizes TonB (By similarity). [EXBD_ECO57] Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Publication Abstract from PubMedIn Gram-negative bacteria, outer membrane transporters import nutrients by coupling to an inner membrane protein complex called the Ton complex. The Ton complex consists of TonB, ExbB, and ExbD, and uses the proton motive force at the inner membrane to transduce energy to the outer membrane via TonB. Here, we structurally characterize the Ton complex from Escherichia coli using X-ray crystallography, electron microscopy, double electron-electron resonance (DEER) spectroscopy, and crosslinking. Our results reveal a stoichiometry consisting of a pentamer of ExbB, a dimer of ExbD, and at least one TonB. Electrophysiology studies show that the Ton subcomplex forms pH-sensitive cation-selective channels and provide insight into the mechanism by which it may harness the proton motive force to produce energy. Structural insight into the role of the Ton complex in energy transduction.,Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK Nature. 2016 Sep 21. doi: 10.1038/nature19757. PMID:27654919[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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