5elq: Difference between revisions
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==Crystal structure of the SNX27 PDZ domain bound to the C-terminal DGKzeta PDZ binding motif== | ==Crystal structure of the SNX27 PDZ domain bound to the C-terminal DGKzeta PDZ binding motif== | ||
<StructureSection load='5elq' size='340' side='right' caption='[[5elq]], [[Resolution|resolution]] 1.10Å' scene=''> | <StructureSection load='5elq' size='340' side='right'caption='[[5elq]], [[Resolution|resolution]] 1.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5elq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ELQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ELQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[5elq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ELQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ELQ FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5elq" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5elq" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Sorting nexin|Sorting nexin]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Buffalo rat]] | [[Category: Buffalo rat]] | ||
[[Category: Large Structures]] | |||
[[Category: Clairfeuille, T]] | [[Category: Clairfeuille, T]] | ||
[[Category: Collins, B M]] | [[Category: Collins, B M]] | ||
Revision as of 11:02, 8 January 2020
Crystal structure of the SNX27 PDZ domain bound to the C-terminal DGKzeta PDZ binding motifCrystal structure of the SNX27 PDZ domain bound to the C-terminal DGKzeta PDZ binding motif
Structural highlights
Function[SNX27_RAT] Involved in endocytic trafficking (By similarity). In T lymphocytes, participates in endocytic recycling pathway. Recruits PSCDBP and HT4R to early endosomes (By similarity). Publication Abstract from PubMedRecycling of internalized receptors from endosomal compartments is essential for the receptors' cell-surface homeostasis. Sorting nexin 27 (SNX27) cooperates with the retromer complex in the recycling of proteins containing type I PSD95-Dlg-ZO1 (PDZ)-binding motifs. Here we define specific acidic amino acid sequences upstream of the PDZ-binding motif required for high-affinity engagement of the human SNX27 PDZ domain. However, a subset of SNX27 ligands, such as the beta2 adrenergic receptor and N-methyl-D-aspartate (NMDA) receptor, lack these sequence determinants. Instead, we identified conserved sites of phosphorylation that substitute for acidic residues and dramatically enhance SNX27 interactions. This newly identified mechanism suggests a likely regulatory switch for PDZ interaction and protein transport by the SNX27-retromer complex. Defining this SNX27 binding code allowed us to classify more than 400 potential SNX27 ligands with broad functional implications in signal transduction, neuronal plasticity and metabolite transport. A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex.,Clairfeuille T, Mas C, Chan AS, Yang Z, Tello-Lafoz M, Chandra M, Widagdo J, Kerr MC, Paul B, Merida I, Teasdale RD, Pavlos NJ, Anggono V, Collins BM Nat Struct Mol Biol. 2016 Sep 5. doi: 10.1038/nsmb.3290. PMID:27595347[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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