1zyr: Difference between revisions

Revision as of 18:14, 3 May 2008

Structure of Thermus thermophilus RNA polymerase holoenzyme in complex with the antibiotic streptolydigin

OverviewOverview

We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.

About this StructureAbout this Structure

1ZYR is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation., Tuske S, Sarafianos SG, Wang X, Hudson B, Sineva E, Mukhopadhyay J, Birktoft JJ, Leroy O, Ismail S, Clark AD Jr, Dharia C, Napoli A, Laptenko O, Lee J, Borukhov S, Ebright RH, Arnold E, Cell. 2005 Aug 26;122(4):541-52. PMID:16122422 Page seeded by OCA on Sat May 3 18:14:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1zyr.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1zyr |SIZE=350|CAPTION= <scene name='initialview01'>1zyr</scene>, resolution 3.00&Aring;
+The line below this paragraph, containing "STRUCTURE_1zyr", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STD:STREPTOLYDIGIN'>STD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1zyr|  PDB=1zyr  |  SCENE=  }}
-|RELATEDENTRY=[[1iw7|1IW7]], [[1smy|1SMY]], [[1ynj|1YNJ]], [[1ynn|1YNN]], [[1k83|1K83]], [[1i6h|1I6H]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyr OCA], [http://www.ebi.ac.uk/pdbsum/1zyr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zyr RCSB]</span>
-}}
 '''Structure of Thermus thermophilus RNA polymerase holoenzyme in complex with the antibiotic streptolydigin'''
@@ Line 43: / Line 40: @@
 [[Category: Tuske, S.]]
 [[Category: Wang, X.]]
-[[Category: holoenzyme]]
+[[Category: Holoenzyme]]
-[[Category: rna polymerase]]
+[[Category: Rna polymerase]]
-[[Category: streptolydigin]]
+[[Category: Streptolydigin]]
-[[Category: transcription]]
+[[Category: Transcription]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:14:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:43:25 2008''