5aa7: Difference between revisions
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==Structural and functional characterization of a chitin-active 15.5 kDa lytic polysaccharide monooxygenase domain from a modular chitinase from Jonesia denitrificans== | ==Structural and functional characterization of a chitin-active 15.5 kDa lytic polysaccharide monooxygenase domain from a modular chitinase from Jonesia denitrificans== | ||
<StructureSection load='5aa7' size='340' side='right' caption='[[5aa7]], [[Resolution|resolution]] 1.55Å' scene=''> | <StructureSection load='5aa7' size='340' side='right'caption='[[5aa7]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5aa7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"listeria_l26"_sohier_et_al.1948 "listeria l26" sohier et al.1948]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AA7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5aa7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"listeria_l26"_sohier_et_al.1948 "listeria l26" sohier et al.1948]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AA7 FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5aa7" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5aa7" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Chitinase 3D structures|Chitinase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Listeria l26 sohier et al 1948]] | [[Category: Listeria l26 sohier et al 1948]] | ||
[[Category: Chitinase]] | [[Category: Chitinase]] | ||
[[Category: Large Structures]] | |||
[[Category: Choudhary, S]] | [[Category: Choudhary, S]] | ||
[[Category: Dalhus, B]] | [[Category: Dalhus, B]] | ||
Revision as of 10:59, 4 March 2020
Structural and functional characterization of a chitin-active 15.5 kDa lytic polysaccharide monooxygenase domain from a modular chitinase from Jonesia denitrificansStructural and functional characterization of a chitin-active 15.5 kDa lytic polysaccharide monooxygenase domain from a modular chitinase from Jonesia denitrificans
Structural highlights
Publication Abstract from PubMedLytic polysaccharide monooxygenases (LPMOs) boost enzymatic depolymerization of recalcitrant polysaccharides, such as chitin and cellulose. We have studied a chitin-active LPMO domain (JdLPMO10A) that is considerably smaller (15.5 kDa) than all structurally characterized LPMOs so far and that is part of a modular protein containing a GH18 chitinase. The 1.55 A resolution structure revealed deletions of interacting loops that protrude from the core beta-sandwich scaffold in larger LPMO10s. Despite these deletions, the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved. JdLPMO10A may represent a minimal scaffold needed to catalyse the powerful LPMO reaction. Structural and functional characterization of a small chitin-active lytic polysaccharide monooxygenase domain of a multi-modular chitinase from Jonesia denitrificans.,Mekasha S, Forsberg Z, Dalhus B, Bacik JP, Choudhary S, Schmidt-Dannert C, Vaaje-Kolstad G, Eijsink VG FEBS Lett. 2016 Jan;590(1):34-42. doi: 10.1002/1873-3468.12025. Epub 2015 Dec 28. PMID:26763108[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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