1zs4: Difference between revisions

Revision as of 18:00, 3 May 2008

Structure of bacteriophage lambda cII protein in complex with DNA

OverviewOverview

The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element. Here, we present the X-ray crystal structure of cII alone (2.8 A resolution) and in complex with its DNA operator from P(RE) (1.7 A resolution). The structures provide a basis for modeling of the activation complex with the RNA polymerase holoenzyme, and point to the key role for the RNA polymerase alpha subunit C-terminal domain (alphaCTD) in cII-dependent activation, which forms a bridge of protein/protein interactions between cII and the RNA polymerase sigma subunit. The model makes specific predictions for protein/protein interactions between cII and alphaCTD, and between alphaCTD and sigma, which are supported by previous genetic studies.

About this StructureAbout this Structure

1ZS4 is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of bacteriophage lambda cII and its DNA complex., Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA, Mol Cell. 2005 Jul 22;19(2):259-69. PMID:16039594 Page seeded by OCA on Sat May 3 18:00:17 2008

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OCA

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 [[Image:1zs4.gif|left|200px]]
- {{Structure
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-}}
 '''Structure of bacteriophage lambda cII protein in complex with DNA'''
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 [[Category: Kim, Y.]]
 [[Category: Maxwell, K L.]]
-[[Category: helix-turn-helix]]
+[[Category: Helix-turn-helix]]
-[[Category: transcription activator]]
+[[Category: Transcription activator]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:00:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:40:53 2008''