1zo3: Difference between revisions

Revision as of 17:52, 3 May 2008

The P-site and P/E-site tRNA structures fitted to P/I site codon.

OverviewOverview

The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:15935757 Page seeded by OCA on Sat May 3 17:52:03 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1zo3.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1zo3 |SIZE=350|CAPTION= <scene name='initialview01'>1zo3</scene>
+The line below this paragraph, containing "STRUCTURE_1zo3", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1zo3|  PDB=1zo3  |  SCENE=  }}
-|RELATEDENTRY=[[1zo1|1ZO1]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zo3 OCA], [http://www.ebi.ac.uk/pdbsum/1zo3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zo3 RCSB]</span>
-}}
 '''The P-site and P/E-site tRNA structures fitted to P/I site codon.'''
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 ==About this Structure==
-ZO3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA].
 ==Reference==
 The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15935757 15935757]
-[[Category: Escherichia coli]]
-[[Category: Protein complex]]
 [[Category: Allen, G S.]]
 [[Category: Ehrenberg, M.]]
@@ Line 30: / Line 25: @@
 [[Category: Gursky, R.]]
 [[Category: Zavialov, A.]]
-[[Category: cryo-eletron microscopy]]
+[[Category: Cryo-eletron microscopy]]
-[[Category: e. coli]]
+[[Category: E. coli]]
-[[Category: initiation factor]]
+[[Category: Initiation factor]]
-[[Category: initiation of protein synthesis]]
+[[Category: Initiation of protein synthesis]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:52:03 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:39:24 2008''