5bv0: Difference between revisions
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==Crystal Structure of a Complex Between the SNARE Nyv1 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum== | ==Crystal Structure of a Complex Between the SNARE Nyv1 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum== | ||
<StructureSection load='5bv0' size='340' side='right' caption='[[5bv0]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='5bv0' size='340' side='right'caption='[[5bv0]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5bv0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50] and [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BV0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5bv0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50] and [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BV0 FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5bv0" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5bv0" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Vacuolar protein sorting-associated protein|Vacuolar protein sorting-associated protein]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Cbs 144 50]] | [[Category: Cbs 144 50]] | ||
[[Category: Chatd]] | [[Category: Chatd]] | ||
[[Category: Large Structures]] | |||
[[Category: Baker, R W]] | [[Category: Baker, R W]] | ||
[[Category: Hughson, F M]] | [[Category: Hughson, F M]] | ||
Revision as of 14:07, 25 December 2019
Crystal Structure of a Complex Between the SNARE Nyv1 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilumCrystal Structure of a Complex Between the SNARE Nyv1 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum
Structural highlights
Publication Abstract from PubMedFusion of intracellular transport vesicles requires soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18-family (SM) proteins. Membrane-bridging SNARE complexes are critical for fusion, but their spontaneous assembly is inefficient and may require SM proteins in vivo. We report x-ray structures of Vps33, the SM subunit of the yeast homotypic fusion and vacuole protein-sorting (HOPS) complex, bound to two individual SNAREs. The two SNAREs, one from each membrane, are held in the correct orientation and register for subsequent complex assembly. Vps33 and potentially other SM proteins could thus act as templates for generating partially zipped SNARE assembly intermediates. HOPS was essential to mediate SNARE complex assembly at physiological SNARE concentrations. Thus, Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition. A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.,Baker RW, Jeffrey PD, Zick M, Phillips BP, Wickner WT, Hughson FM Science. 2015 Sep 4;349(6252):1111-4. doi: 10.1126/science.aac7906. PMID:26339030[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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