1zim: Difference between revisions

Revision as of 17:40, 3 May 2008

GCN4-LEUCINE ZIPPER CORE MUTANT ASN16GLN IN THE TRIMERIC STATE

OverviewOverview

A conserved asparagine (Asn 16) buried in the interface of the GCN4 leucine zipper selectively favours the parallel, dimeric, coiled-coil structure. To test if other polar residues confer oligomerization specificity, the structural effects of Gln and Lys substitutions for Asn 16 were characterized. Like the wild-type peptide, the Asn 16Lys mutant formed exclusively dimers. In contrast, Gln 16, despite its chemical similarity to Asn, allowed the peptide to form both dimers and trimers. The Gln 16 side chain was accommodated by qualitatively different interactions in the dimer and trimer crystal structures. These findings demonstrate that the structural selectivity of polar residues results not only from the burial of polar atoms, but also depends on the complementarity of the side-chain stereochemistry with the surrounding structural environment.

About this StructureAbout this Structure

1ZIM is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Buried polar residues and structural specificity in the GCN4 leucine zipper., Gonzalez L Jr, Woolfson DN, Alber T, Nat Struct Biol. 1996 Dec;3(12):1011-8. PMID:8946854 Page seeded by OCA on Sat May 3 17:40:19 2008

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OCA

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 [[Image:1zim.jpg|left|200px]]
- {{Structure
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-}}
 '''GCN4-LEUCINE ZIPPER CORE MUTANT ASN16GLN IN THE TRIMERIC STATE'''
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 [[Category: Junior, L Gonzalez.]]
 [[Category: Woolfson, D N.]]
-[[Category: activator]]
+[[Category: Activator]]
-[[Category: amino-acid biosynthesis]]
+[[Category: Amino-acid biosynthesis]]
-[[Category: coiled coil]]
+[[Category: Coiled coil]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: leucine zipper]]
+[[Category: Leucine zipper]]
-[[Category: nuclear protein]]
+[[Category: Nuclear protein]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:40:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:08 2008''