6b9r: Difference between revisions

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-'''Unreleased structure'''
-The entry 6b9r is ON HOLD  until Paper Publication
+==Streptomyces albus HEPD with substrate 2-hydroxyethylphosphonate (2-HEP) and Fe(II) bound==
+<StructureSection load='6b9r' size='340' side='right' caption='[[6b9r]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6b9r]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B9R FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2HE:(2-HYDROXYETHYL)PHOSPHONIC+ACID'>2HE</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b9r OCA], [http://pdbe.org/6b9r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b9r RCSB], [http://www.ebi.ac.uk/pdbsum/6b9r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b9r ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Methylphosphonate synthase (MPnS) produces methylphosphonate, a metabolic precursor to methane in the upper ocean. Here, we determine a 2.35-angstrom resolution structure of MPnS and discover that it has an unusual 2-histidine-1-glutamine iron-coordinating triad. We further solve the structure of a related enzyme, hydroxyethylphosphonate dioxygenase from Streptomyces albus (SaHEPD), and find that it displays the same motif. SaHEPD can be converted into an MPnS by mutation of glutamine-adjacent residues, identifying the molecular requirements for methylphosphonate synthesis. Using these sequence markers, we find numerous putative MPnSs in marine microbiomes and confirm that MPnS is present in the abundant Pelagibacter ubique. The ubiquity of MPnS-containing microbes supports the proposal that methylphosphonate is a source of methane in the upper, aerobic ocean, where phosphorus-starved microbes catabolize methylphosphonate for its phosphorus.
-Authors:
+Structural basis for methylphosphonate biosynthesis.,Born DA, Ulrich EC, Ju KS, Peck SC, van der Donk WA, Drennan CL Science. 2017 Dec 8;358(6368):1336-1339. doi: 10.1126/science.aao3435. PMID:29217579<ref>PMID:29217579</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6b9r" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Born, D A]]
+[[Category: Drennan, C L]]
+[[Category: Hydroxymethylphosphonate]]
+[[Category: Iron]]
+[[Category: Oxidoreductase]]
+[[Category: Phosphonate]]