1z0h: Difference between revisions

Revision as of 17:02, 3 May 2008

N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B

OverviewOverview

Botulinum neurotoxins comprise seven distinct serotypes (A-G) produced by Clostridium botulinum. The crystal structure of the binding domain of the botulinum neurotoxin type B (BBHc) has been determined to 2A resolution. The overall structure of BBHc is well ordered and similar to that of the binding domain of the holotoxin. However, significant structural changes occur at what would be the interface of translocation and binding domains of the holotoxin. The loop 911-924 shows a maximum displacement of 14.8A at the farthest point. The N-terminal helix reorients and moves by 19.5A from its original position. BBHc is compared with the binding domain of the holotoxin of botulinum type A and B, and the tetanus C-fragment to characterize the heavy chain-carbohydrate interactions. The probable reasons for different binding affinity of botulinum and tetanus toxins are discussed.

About this StructureAbout this Structure

1Z0H is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.

ReferenceReference

N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B., Jayaraman S, Eswaramoorthy S, Ahmed SA, Smith LA, Swaminathan S, Biochem Biophys Res Commun. 2005 Apr 29;330(1):97-103. PMID:15781237 Page seeded by OCA on Sat May 3 17:02:16 2008

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OCA

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 [[Image:1z0h.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1z0h", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])
+-->
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z0h OCA], [http://www.ebi.ac.uk/pdbsum/1z0h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z0h RCSB]</span>
-}}
 '''N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B'''
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 [[Category: Smith, L A.]]
 [[Category: Swaminathan, S.]]
-[[Category: binding domain]]
+[[Category: Binding domain]]
-[[Category: clostridium botulinum]]
+[[Category: Clostridium botulinum]]
-[[Category: ganglioside]]
+[[Category: Ganglioside]]
-[[Category: x-ray crystallography]]
+[[Category: X-ray crystallography]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:02:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:27:15 2008''