2hhc: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of fucosyltransferase NodZ from Bradyrhizobium== | ==Crystal structure of fucosyltransferase NodZ from Bradyrhizobium== | ||
<StructureSection load='2hhc' size='340' side='right' caption='[[2hhc]], [[Resolution|resolution]] 1.54Å' scene=''> | <StructureSection load='2hhc' size='340' side='right'caption='[[2hhc]], [[Resolution|resolution]] 1.54Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2hhc]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2hhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brasw Brasw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HHC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nodZ ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nodZ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=133505 BRASW])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hhc OCA], [https://pdbe.org/2hhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hhc RCSB], [https://www.ebi.ac.uk/pdbsum/2hhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hhc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
| Line 12: | Line 12: | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hhc_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hhc_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 27: | Line 27: | ||
</div> | </div> | ||
<div class="pdbe-citations 2hhc" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2hhc" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[NodS|NodS]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 32: | Line 35: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Brasw]] | [[Category: Brasw]] | ||
[[Category: Large Structures]] | |||
[[Category: Brzezinski, K]] | [[Category: Brzezinski, K]] | ||
[[Category: Bujacz, G]] | [[Category: Bujacz, G]] | ||
Revision as of 10:39, 17 March 2021
Crystal structure of fucosyltransferase NodZ from BradyrhizobiumCrystal structure of fucosyltransferase NodZ from Bradyrhizobium
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain. High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor.,Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:17762900[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
| ||||||||||||||||||