1ylb: Difference between revisions
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'''NMR solution structure of the reduced spinach plastocyanin''' | '''NMR solution structure of the reduced spinach plastocyanin''' | ||
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[[Category: Musiani, F.]] | [[Category: Musiani, F.]] | ||
[[Category: Semenov, A Y.]] | [[Category: Semenov, A Y.]] | ||
[[Category: | [[Category: Blue-copper protein]] | ||
[[Category: | [[Category: Electron-transfer]] | ||
[[Category: Photosynthesis]] | |||
[[Category: | [[Category: Plastocyanin]] | ||
[[Category: | [[Category: Spinach]] | ||
[[Category: | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:27:53 2008'' | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 16:27, 3 May 2008
NMR solution structure of the reduced spinach plastocyanin
OverviewOverview
In oxygenic photosynthesis, plastocyanin shuttles electrons between the membrane-bound complexes cytochrome b6f and photosystem I. The homologous complex between cytochrome f and plastocyanin, both from spinach, is the object of this study. The solution structure of the reduced spinach plastocyanin was determined using high field NMR spectroscopy, whereas the model structure of oxidized cytochrome f was obtained by homology modeling calculations and molecular dynamics. The model structure of the intermolecular complex was calculated using the program AUTODOCK, taking into account biological information obtained from mutagenesis experiments. The best electron transfer pathway from the heme group of cytochrome f to the copper ion of plastocyanin was calculated using the program HARLEM, obtaining a coupling decay value of 1.8 x 10(-4). Possible mechanisms of interaction and electron transfer between plastocyanin and cytochrome f were discussed considering the possible formation of a supercomplex that associates one cytochrome b6f, one photosystem I, and one plastocyanin.
About this StructureAbout this Structure
1YLB is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach., Musiani F, Dikiy A, Semenov AY, Ciurli S, J Biol Chem. 2005 May 13;280(19):18833-41. Epub 2005 Feb 3. PMID:15691836 Page seeded by OCA on Sat May 3 16:27:53 2008