1yal: Difference between revisions

Revision as of 16:04, 3 May 2008

CARICA PAPAYA CHYMOPAPAIN AT 1.7 ANGSTROMS RESOLUTION

OverviewOverview

The X-ray structure of chymopapain, a cysteine proteinase isolated from the latex of the fruits of Carica papaya L., has been determined by molecular replacement methods and refined to a conventional R factor of 0.19 for all observed reflections in the range from 9.5 to 1.7 A resolution. The crystals used in this study contained a unique molecular species of chymopapain with two moles of thiomethyl attached to the two free cysteines per mole of enzyme. A comparison is made with the other known papaya proteinase X-ray structures: papain, caricain, and glycyl endopeptidase. Their backbone conformations are extremely similar except for two loop regions. Both regions are located at the surface of the protein and far away of the active site cleft. In each X-ray structure the same water network was found at the interface between the two domains of the enzyme. A close examination of the active site groove showed that the specificity restrictions dictated by the S2 subsite did not differ significantly among the four proteinases.

About this StructureAbout this Structure

1YAL is a Single protein structure of sequence from Carica papaya. Full crystallographic information is available from OCA.

ReferenceReference

Structure of chymopapain at 1.7 A resolution., Maes D, Bouckaert J, Poortmans F, Wyns L, Looze Y, Biochemistry. 1996 Dec 17;35(50):16292-8. PMID:8973203 Page seeded by OCA on Sat May 3 16:04:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1yal.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1yal", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SCH:S-METHYL+THIOCYSTEINE+GROUP'>SCH</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymopapain Chymopapain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.6 3.4.22.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1yal|  PDB=1yal  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yal OCA], [http://www.ebi.ac.uk/pdbsum/1yal PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yal RCSB]</span>
-}}
 '''CARICA PAPAYA CHYMOPAPAIN AT 1.7 ANGSTROMS RESOLUTION'''
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 [[Category: Poortmans, F.]]
 [[Category: Wyns, L.]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: thiol protease]]
+[[Category: Thiol protease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:04:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:00:56 2008''