1xqi: Difference between revisions

Revision as of 15:22, 3 May 2008

Crystal Structure Analysis of an NDP kinase from Pyrobaculum aerophilum

OverviewOverview

Nucleoside diphosphate (NDP) kinases are ubiquitous enzymes that transfer gamma-phosphates from nucleoside triphosphates to nucleoside diphosphates via a ping-pong mechanism. The important role of this large family of enzymes in controlling cellular functions and developmental processes along with their crystallizability has made them good candidates for structural studies. We recently determined the structure of an evolved version of an NDP kinase from Pyrobaculum aerophilum, an extreme thermophile. This NDP kinase has similarity to the 42 other NDP kinases deposited in the Protein Data Bank (PDB) but differs significantly in sequence, structure, and biophysical properties. The P. aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66-100 and 156-165. We show that deletion mutants of the P. aerophilum NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the P. aerophilum NDP kinase is related to the presence of these two inserts.

About this StructureAbout this Structure

1XQI is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.

ReferenceReference

Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum., Pedelacq JD, Waldo GS, Cabantous S, Liong EC, Terwilliger TC, Protein Sci. 2005 Oct;14(10):2562-73. PMID:16195547 Page seeded by OCA on Sat May 3 15:22:28 2008

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OCA

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 [[Image:1xqi.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span>
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-|GENE= ndk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13773 Pyrobaculum aerophilum])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xqi OCA], [http://www.ebi.ac.uk/pdbsum/1xqi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xqi RCSB]</span>
-}}
 '''Crystal Structure Analysis of an NDP kinase from Pyrobaculum aerophilum'''
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 [[Category: Terwilliger, T C.]]
 [[Category: Waldo, G S.]]
-[[Category: alpha/beta sandwich]]
+[[Category: Alpha/beta sandwich]]
-[[Category: ferredoxin fold]]
+[[Category: Ferredoxin fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:22:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:53:18 2008''