5xt2: Difference between revisions

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-'''Unreleased structure'''
-The entry 5xt2 is ON HOLD  until Paper Publication
+==Crystal structures of full-length FixJ from B. japonicum crystallized in space group P212121==
+<StructureSection load='5xt2' size='340' side='right' caption='[[5xt2]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5xt2]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XT2 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xso|5xso]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xt2 OCA], [http://pdbe.org/5xt2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xt2 RCSB], [http://www.ebi.ac.uk/pdbsum/5xt2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xt2 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The symbiotic nitrogen-fixing bacterium Bradyrhizobium japonicum is critical to the agro-industrial production of soybean because it enables the production of high yields of soybeans with little use of nitrogenous fertilizers. The FixL and FixJ two-component system (TCS) of this bacterium ensures that nitrogen fixation is only stimulated under conditions of low oxygen. When it is not bound to oxygen, the histidine kinase FixL undergoes autophosphorylation and transfers phosphate from adenosine triphosphate (ATP) to the response regulator FixJ, which, in turn, stimulates the expression of genes required for nitrogen fixation. We purified full-length B. japonicum FixL and FixJ proteins and defined their structures individually and in complex using small-angle x-ray scattering, crystallographic, and in silico modeling techniques. Comparison of active and inactive forms of FixL suggests that intramolecular signal transduction is driven by local changes in the sensor domain and in the coiled-coil region connecting the sensor and histidine kinase domains. We also found that FixJ exhibits conformational plasticity not only in the monomeric state but also in tetrameric complexes with FixL during phosphotransfer. This structural characterization of a complete TCS contributes both a mechanistic and evolutionary understanding to TCS signal relay, specifically in the context of the control of nitrogen fixation in root nodules.
-Authors: Nishizono, Y., Hisano, T., Shiro, Y., Sawai, H., Wright, G.S.A., Saeki, A., Hikima, T., Nakamura, H., Yamamoto, M., Antonyuk, S.V., Hasnain, S.S.
+Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system.,Wright GSA, Saeki A, Hikima T, Nishizono Y, Hisano T, Kamaya M, Nukina K, Nishitani H, Nakamura H, Yamamoto M, Antonyuk SV, Hasnain SS, Shiro Y, Sawai H Sci Signal. 2018 Apr 10;11(525). pii: 11/525/eaaq0825. doi:, 10.1126/scisignal.aaq0825. PMID:29636388<ref>PMID:29636388</ref>
-Description: Crystal structures of full-length FixJ from B. japonicum crystallized in space group P212121
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5xt2" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Antonyuk, S V]]
+[[Category: Hasnain, S S]]
+[[Category: Hikima, T]]
+[[Category: Hisano, T]]
+[[Category: Nakamura, H]]
+[[Category: Nishizono, Y]]
+[[Category: Saeki, A]]
+[[Category: Sawai, H]]
 [[Category: Shiro, Y]]
+[[Category: Wright, G S.A]]
 [[Category: Yamamoto, M]]
-[[Category: Hisano, T]]
+[[Category: Dna binding protein]]
-[[Category: Hikima, T]]
+[[Category: Fixj/narl family]]
-[[Category: Antonyuk, S.V]]
+[[Category: Full-length]]
-[[Category: Sawai, H]]
+[[Category: Response regulater]]
-[[Category: Saeki, A]]
+[[Category: Unphosphorylated monomeric state]]
-[[Category: Wright, G.S.A]]
-[[Category: Hasnain, S.S]]
-[[Category: Nishizono, Y]]
-[[Category: Nakamura, H]]