1kpc: Difference between revisions

Revision as of 18:46, 12 November 2007

PKCI-1-APO+ZINC

OverviewOverview

The three-dimensional structure of protein kinase C interacting protein 1, (PKCI-1) has been solved to high resolution by x-ray crystallography using, single isomorphous replacement with anomalous scattering. The gene, encoding human PKCI-1 was cloned from a cDNA library by using a partial, sequence obtained from interactions identified in the yeast two-hybrid, system between PKCI-1 and the regulatory domain of protein kinase C-beta., The PKCI-1 protein was expressed in Pichia pastoris as a dimer of two, 13.7-kDa polypeptides. PKCI-1 is a member of the HIT family of proteins, shown by sequence identity to be conserved in a broad range of organisms, including mycoplasma, plants, and humans. Despite the ubiquity of this, protein sequence in nature, no distinct function has been shown for the, protein product in vitro or in vivo. The PKCI-1 protomer has an alpha+beta, meander fold containing a five-stranded antiparallel sheet and two, helices. Two protomers come together to form a 10-stranded antiparallel, sheet with extensive contacts between a helix and carboxy terminal amino, acids of a protomer with the corresponding amino acids in the other, protomer. PKCI-1 has been shown to interact specifically with zinc. The, three-dimensional structure has been solved in the presence and absence of, zinc and in two crystal forms. The structure of human PKCI-1 provides a, model of this family of proteins which suggests a stable fold conserved, throughout nature.

About this StructureAbout this Structure

1KPC is a Single protein structure of sequence from Homo sapiens. Structure known Active Sites: ZNA, ZNB, ZNC and ZND. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins., Lima CD, Klein MG, Weinstein IB, Hendrickson WA, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5357-62. PMID:8643579

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	[[Category: zinc binding protein]]		[[Category: zinc binding protein]]

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