1x9l: Difference between revisions

Revision as of 08:13, 13 April 2008

Solution structure of CuI-DR1885 from Deinococcus Radiodurans

OverviewOverview

Sco1 and Cox17 are accessory proteins required for the correct assembly of eukaryotic cytochrome c oxidase. At variance with Sco1, Cox17 orthologs are found only in eukaryotes. We browsed bacterial genomes to search proteins functionally equivalent to Cox17, and we identified a class of proteins of unknown function displaying a conserved gene neighborhood to bacterial Sco1 genes, all sharing a potential metal binding motif H(M)X10MX21HXM. Two members of this group, DR1885 from Deinococcus radiodurans and CC3502 from Caulobacter crescentus, were expressed, and their interaction with copper was investigated. The solution structure and extended x-ray absorption fine structure data on the former protein reveal that the protein binds copper(I) through a histidine and three Mets in a cupredoxin-like fold. The surface location of the copper-binding site as well as the type of coordination are well poised for metal transfer chemistry, suggesting that DR1885 might transfer copper, taking the role of Cox17 in bacteria. On the basis of our results, a possible pathway for copper delivery to the Cu(A) center in bacteria is proposed.

About this StructureAbout this Structure

1X9L is a Single protein structure of sequence from Deinococcus radiodurans r1. Full crystallographic information is available from OCA.

ReferenceReference

A copper(I) protein possibly involved in the assembly of CuA center of bacterial cytochrome c oxidase., Banci L, Bertini I, Ciofi-Baffoni S, Katsari E, Katsaros N, Kubicek K, Mangani S, Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):3994-9. Epub 2005 Mar 7. PMID:15753304 Page seeded by OCA on Sun Apr 13 08:13:47 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1x9l.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1x9l |SIZE=350|CAPTION= <scene name='initialview01'>1x9l</scene>
+The line below this paragraph, containing "STRUCTURE_1x9l", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1x9l|  PDB=1x9l  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x9l OCA], [http://www.ebi.ac.uk/pdbsum/1x9l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x9l RCSB]</span>
-}}
 '''Solution structure of CuI-DR1885 from Deinococcus Radiodurans'''
@@ Line 32: / Line 29: @@
 [[Category: Kubicek, K.]]
 [[Category: SPINE, Structural Proteomics in Europe.]]
-[[Category: copper binding protein]]
+[[Category: Copper binding protein]]
-[[Category: deinococcus radioduran]]
+[[Category: Deinococcus radioduran]]
-[[Category: holo-form]]
+[[Category: Holo-form]]
-[[Category: nmr]]
+[[Category: Nmr]]
-[[Category: solution structure]]
+[[Category: Solution structure]]
-[[Category: spine]]
+[[Category: Spine]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: structural proteomics in europe]]
+[[Category: Structural proteomics in europe]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:13:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:46:39 2008''