1x35: Difference between revisions

Revision as of 14:28, 3 May 2008

Recombinant T=3 capsid of a site specific mutant of SeMV CP

OverviewOverview

When expressed in Escherichia coli, the recombinant coat protein (rCP) of Sesbania mosaic virus (SeMV) was shown to self-assemble into T = 3 capsids encapsidating CP mRNA and 23S rRNA derived from the host. Expression of CP-P53A, in which a conserved proline at position 53 in the beta-annulus was substituted by alanine (CP-P53A), also produced similar capsids. Purified rCP and CP-P53A particles were crystallized and X-ray crystal structures of their mutant capsids were determined to resolutions of 3.6 and 4.1 A, respectively. As in the native viral CP, the CPs in these recombinant capsids adopt the jelly-roll beta-sandwich fold. The amino-terminal residues of the C subunits alone are ordered and form the beta-annulus structure at the quasi-sixfold axes. A characteristic bend in the beta-annulus remains unaffected in CP-P53A. The quasi-threefold interfaces of the capsids harbour calcium ions coordinated by ligands from the adjacent threefold-related subunits in a geometry that is analogous to that observed in the native capsid. Taken together with studies on deletion and substitution mutants of SeMV CP, these results suggest the possibility that the beta-annulus and nucleic acid-mediated interactions may be less important for the assembly of sobemoviruses than previously envisaged.

About this StructureAbout this Structure

1X35 is a Single protein structure of sequence from Sesbania mosaic virus. Full crystallographic information is available from OCA.

ReferenceReference

Structural studies on recombinant T = 3 capsids of Sesbania mosaic virus coat protein mutants., Sangita V, Lokesh GL, Satheshkumar PS, Saravanan V, Vijay CS, Savithri HS, Murthy MR, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1402-5. Epub 2005, Sep 28. PMID:16204893 Page seeded by OCA on Sat May 3 14:28:02 2008

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OCA

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 [[Image:1x35.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1x35 |SIZE=350|CAPTION= <scene name='initialview01'>1x35</scene>, resolution 4.10&Aring;
+The line below this paragraph, containing "STRUCTURE_1x35", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1x35|  PDB=1x35  |  SCENE=  }}
-|RELATEDENTRY=[[1smv|1SMV]], [[1x33|1X33]], [[1x34|1X34]], [[1x36|1X36]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x35 OCA], [http://www.ebi.ac.uk/pdbsum/1x35 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x35 RCSB]</span>
-}}
 '''Recombinant T=3 capsid of a site specific mutant of SeMV CP'''
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 [[Category: Savithri, H S.]]
 [[Category: Vijay, C S.]]
-[[Category: beta-annulus]]
+[[Category: Beta-annulus]]
-[[Category: icosahedral virus]]
+[[Category: Icosahedral virus]]
-[[Category: t=3 capsid]]
+[[Category: T=3 capsid]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 14:28:02 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:44:12 2008''