1wt2: Difference between revisions

Revision as of 14:06, 3 May 2008

Mutant human ABO(H) blood group glycosyltransferase A with bound UDP and inhibitor

OverviewOverview

The human ABO(H) blood group antigens are carbohydrate structures generated by glycosyltransferase enzymes. Glycosyltransferase A (GTA) uses UDP-GalNAc as a donor to transfer a monosaccharide residue to Fuc alpha1-2Gal beta-R (H)-terminating acceptors. Similarly, glycosyltransferase B (GTB) catalyzes the transfer of a monosaccharide residue from UDP-Gal to the same acceptors. These are highly homologous enzymes differing in only four of 354 amino acids, Arg/Gly-176, Gly/Ser-235, Leu/Met-266, and Gly/Ala-268. Blood group O usually stems from the expression of truncated inactive forms of GTA or GTB. Recently, an O(2) enzyme was discovered that was a full-length form of GTA with three mutations, P74S, R176G, and G268R. We showed previously that the R176G mutation increased catalytic activity with minor effects on substrate binding. Enzyme kinetics and high resolution structural studies of mutant enzymes based on the O(2) blood group transferase reveal that whereas the P74S mutation in the stem region of the protein does not appear to play a role in enzyme inactivation, the G268R mutation completely blocks the donor GalNAc-binding site leaving the acceptor binding site unaffected.

About this StructureAbout this Structure

1WT2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the inactivity of human blood group O2 glycosyltransferase., Lee HJ, Barry CH, Borisova SN, Seto NO, Zheng RB, Blancher A, Evans SV, Palcic MM, J Biol Chem. 2005 Jan 7;280(1):525-9. Epub 2004 Oct 8. PMID:15475562 Page seeded by OCA on Sat May 3 14:06:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1wt2.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1wt2", creates the "Structure Box" on the page.
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-|LIGAND= <scene name='pdbligand=DLG:HEXYL+3-DEOXY-ALPHA-L-RIBO-HEXOPYRANOSIDE'>DLG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=UDP:URIDINE-5&#39;-DIPHOSPHATE'>UDP</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycoprotein-fucosylgalactoside_alpha-N-acetylgalactosaminyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.40 2.4.1.40] </span>
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-|DOMAIN=
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-|RELATEDENTRY=[[1wsz|1WSZ]], [[1wt0|1WT0]], [[1wt1|1WT1]], [[1wt3|1WT3]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wt2 OCA], [http://www.ebi.ac.uk/pdbsum/1wt2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wt2 RCSB]</span>
-}}
 '''Mutant human ABO(H) blood group glycosyltransferase A with bound UDP and inhibitor'''
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 [[Category: Seto, N O.L.]]
 [[Category: Zheng, R B.]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 14:06:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:40:36 2008''