1wac: Difference between revisions

Revision as of 13:22, 3 May 2008

BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE

OverviewOverview

The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.

About this StructureAbout this Structure

1WAC is a Single protein structure of sequence from Pseudomonas phage phi6. Full crystallographic information is available from OCA.

ReferenceReference

Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773 Page seeded by OCA on Sat May 3 13:22:49 2008

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OCA

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 '''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''
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 [[Category: Salgado, P S.]]
 [[Category: Stuart, D I.]]
-[[Category: phi6 rna-dependent rna polymerase]]
+[[Category: Phi6 rna-dependent rna polymerase]]
-[[Category: transcription]]
+[[Category: Transcription]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:22:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:28 2008''