1vzy: Difference between revisions

Revision as of 12:58, 3 May 2008

CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS HSP33

OverviewOverview

The bacterial heat shock protein Hsp33 is a redox-regulated chaperone activated by oxidative stress. In response to oxidation, four cysteines within a Zn2+ binding C-terminal domain form two disulfide bonds with concomitant release of the metal. This leads to the formation of the biologically active Hsp33 dimer. The crystal structure of the N-terminal domain of the E. coli protein has been reported, but neither the structure of the Zn2+ binding motif nor the nature of its regulatory interaction with the rest of the protein are known. Here we report the crystal structure of the full-length B. subtilis Hsp33 in the reduced form. The structure of the N-terminal, dimerization domain is similar to that of the E. coli protein, although there is no domain swapping. The Zn2+ binding domain is clearly resolved showing the details of the tetrahedral coordination of Zn2+ by four thiolates. We propose a structure-based activation pathway for Hsp33.

About this StructureAbout this Structure

1VZY is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of the reduced, Zn2+-bound form of the B. subtilis Hsp33 chaperone and its implications for the activation mechanism., Janda I, Devedjiev Y, Derewenda U, Dauter Z, Bielnicki J, Cooper DR, Graf PC, Joachimiak A, Jakob U, Derewenda ZS, Structure. 2004 Oct;12(10):1901-7. PMID:15458638 Page seeded by OCA on Sat May 3 12:58:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1vzy.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1vzy |SIZE=350|CAPTION= <scene name='initialview01'>1vzy</scene>, resolution 1.97&Aring;
+The line below this paragraph, containing "STRUCTURE_1vzy", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=ZNB:Act+Binding+Site+For+Chain+A'>ZNB</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00498 Hsp33]</span>
+{{STRUCTURE_1vzy|  PDB=1vzy  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzy OCA], [http://www.ebi.ac.uk/pdbsum/1vzy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vzy RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS HSP33'''
@@ Line 34: / Line 31: @@
 [[Category: Joachimiak, A.]]
 [[Category: MCSG, Midwest Center for Structural Genomics.]]
-[[Category: heat shock protein,crystal engineering,molecular chaperone,redox-active center,psi,protein structure initiative,mcsg,midwest center for structural genomic]]
+[[Category: Heat shock protein,crystal engineering,molecular chaperone,redox-active center,psi,protein structure initiative,mcsg,midwest center for structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:58:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:29:13 2008''