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==Sulfolobus solfataricus HJC mutants==
==Sulfolobus solfataricus HJC mutants==
<StructureSection load='4tkd' size='340' side='right' caption='[[4tkd]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
<StructureSection load='4tkd' size='340' side='right'caption='[[4tkd]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4tkd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TKD FirstGlance]. <br>
<table><tr><td colspan='2'>[[4tkd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TKD FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tkk|4tkk]]</td></tr>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tkk|4tkk]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hjc, SSO0575, ORF-c21_024 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tkd OCA], [http://pdbe.org/4tkd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tkd RCSB], [http://www.ebi.ac.uk/pdbsum/4tkd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tkd ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tkd OCA], [http://pdbe.org/4tkd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tkd RCSB], [http://www.ebi.ac.uk/pdbsum/4tkd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tkd ProSAT]</span></td></tr>
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== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/HJC_SULSO HJC_SULSO]] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>   
[[http://www.uniprot.org/uniprot/HJC_SULSO HJC_SULSO]] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>   
==See Also==
*[[Resolvase|Resolvase]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Crossover junction endodeoxyribonuclease]]
[[Category: Crossover junction endodeoxyribonuclease]]
[[Category: Large Structures]]
[[Category: Sacs2]]
[[Category: Bond, C S]]
[[Category: Bond, C S]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 13:04, 1 May 2019

Sulfolobus solfataricus HJC mutantsSulfolobus solfataricus HJC mutants

Structural highlights

4tkd is a 4 chain structure with sequence from Sacs2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:hjc, SSO0575, ORF-c21_024 (SACS2)
Activity:Crossover junction endodeoxyribonuclease, with EC number 3.1.22.4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HJC_SULSO] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.[1] [2] [3] [4] [5]

See Also

References

  1. ↑ Charlebois RL, Singh RK, Chan-Weiher CC, Allard G, Chow C, Confalonieri F, Curtis B, Duguet M, Erauso G, Faguy D, Gaasterland T, Garrett RA, Gordon P, Jeffries AC, Kozera C, Kushwaha N, Lafleur E, Medina N, Peng X, Penny SL, She Q, St Jean A, van der Oost J, Young F, Zivanovic Y, Doolittle WF, Ragan MA, Sensen CW. Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2. Genome. 2000 Feb;43(1):116-36. PMID:10701121
  2. ↑ Kvaratskhelia M, White MF. Two Holliday junction resolving enzymes in Sulfolobus solfataricus. J Mol Biol. 2000 Apr 7;297(4):923-32. PMID:10736227 doi:http://dx.doi.org/10.1006/jmbi.2000.3624
  3. ↑ Kvaratskhelia M, Wardleworth BN, Norman DG, White MF. A conserved nuclease domain in the archaeal Holliday junction resolving enzyme Hjc. J Biol Chem. 2000 Aug 18;275(33):25540-6. PMID:10940317 doi:http://dx.doi.org/10.1074/jbc.M003420200
  4. ↑ Kvaratskhelia M, Wardleworth BN, Bond CS, Fogg JM, Lilley DM, White MF. Holliday junction resolution is modulated by archaeal chromatin components in vitro. J Biol Chem. 2002 Jan 25;277(4):2992-6. Epub 2001 Nov 14. PMID:11709558 doi:http://dx.doi.org/10.1074/jbc.M109496200
  5. ↑ Dorazi R, Parker JL, White MF. PCNA activates the Holliday junction endonuclease Hjc. J Mol Biol. 2006 Dec 1;364(3):243-7. Epub 2006 Sep 9. PMID:17011573 doi:http://dx.doi.org/10.1016/j.jmb.2006.09.011

4tkd, resolution 2.01Å

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