4znl: Difference between revisions
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==Thermus Phage P74-26 Large Terminase ATPase domain bound to ADP Beryllium Fluoride== | ==Thermus Phage P74-26 Large Terminase ATPase domain bound to ADP Beryllium Fluoride== | ||
<StructureSection load='4znl' size='340' side='right' caption='[[4znl]], [[Resolution|resolution]] 2.07Å' scene=''> | <StructureSection load='4znl' size='340' side='right'caption='[[4znl]], [[Resolution|resolution]] 2.07Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4znl]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZNL FirstGlance]. <br> | <table><tr><td colspan='2'>[[4znl]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_phage_p74-26 Thermus phage p74-26]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZNL FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zni|4zni]], [[4znj|4znj]], [[4znk|4znk]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zni|4zni]], [[4znj|4znj]], [[4znk|4znk]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P74p84 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=466052 Thermus phage P74-26])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4znl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4znl OCA], [http://pdbe.org/4znl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4znl RCSB], [http://www.ebi.ac.uk/pdbsum/4znl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4znl ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4znl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4znl OCA], [http://pdbe.org/4znl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4znl RCSB], [http://www.ebi.ac.uk/pdbsum/4znl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4znl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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</div> | </div> | ||
<div class="pdbe-citations 4znl" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4znl" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Terminase|Terminase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Thermus phage p74-26]] | |||
[[Category: Duffy, C M]] | [[Category: Duffy, C M]] | ||
[[Category: Hayes, J A]] | [[Category: Hayes, J A]] | ||
Revision as of 11:13, 26 June 2019
Thermus Phage P74-26 Large Terminase ATPase domain bound to ADP Beryllium FluorideThermus Phage P74-26 Large Terminase ATPase domain bound to ADP Beryllium Fluoride
Structural highlights
Publication Abstract from PubMedMany viruses package their genomes into procapsids using an ATPase machine that is among the most powerful known biological motors. However, how this motor couples ATP hydrolysis to DNA translocation is still unknown. Here, we introduce a model system with unique properties for studying motor structure and mechanism. We describe crystal structures of the packaging motor ATPase domain that exhibit nucleotide-dependent conformational changes involving a large rotation of an entire subdomain. We also identify the arginine finger residue that catalyzes ATP hydrolysis in a neighboring motor subunit, illustrating that previous models for motor structure need revision. Our findings allow us to derive a structural model for the motor ring, which we validate using small-angle X-ray scattering and comparisons with previously published data. We illustrate the model's predictive power by identifying the motor's DNA-binding and assembly motifs. Finally, we integrate our results to propose a mechanistic model for DNA translocation by this molecular machine. Structure and mechanism of the ATPase that powers viral genome packaging.,Hilbert BJ, Hayes JA, Stone NP, Duffy CM, Sankaran B, Kelch BA Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):E3792-9. doi:, 10.1073/pnas.1506951112. Epub 2015 Jul 6. PMID:26150523[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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