1h83: Difference between revisions
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[[Image:1h83.gif|left|200px]]<br /> | [[Image:1h83.gif|left|200px]]<br /><applet load="1h83" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1h83" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1h83, resolution 1.9Å" /> | caption="1h83, resolution 1.9Å" /> | ||
'''STRUCTURE OF POLYAMINE OXIDASE IN COMPLEX WITH 1,8-DIAMINOOCTANE'''<br /> | '''STRUCTURE OF POLYAMINE OXIDASE IN COMPLEX WITH 1,8-DIAMINOOCTANE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1H83 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with NAG, FAD and DIA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polyamine_oxidase Polyamine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.11 1.5.3.11] | 1H83 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with NAG, FAD and DIA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polyamine_oxidase Polyamine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.11 1.5.3.11] Known structural/functional Sites: <scene name='pdbsite=DIA:Dia Binding Site For Chain A'>DIA</scene>, <scene name='pdbsite=DIB:Dia Binding Site For Chain B'>DIB</scene>, <scene name='pdbsite=DIC:Dia Binding Site For Chain C'>DIC</scene>, <scene name='pdbsite=FAA:Fad Binding Site For Chain A'>FAA</scene>, <scene name='pdbsite=FAB:Fad Binding Site For Chain B'>FAB</scene> and <scene name='pdbsite=FAC:Fad Binding Site For Chain C'>FAC</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H83 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:07:51 2007'' | ||
Revision as of 16:58, 18 December 2007
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STRUCTURE OF POLYAMINE OXIDASE IN COMPLEX WITH 1,8-DIAMINOOCTANE
OverviewOverview
Polyamine oxidase (PAO) carries out the FAD-dependent oxidation of the, secondary amino groups of spermidine and spermine, a key reaction in the, polyamine catabolism. The active site of PAO consists of a 30 A long, U-shaped catalytic tunnel, whose innermost part is located in front of the, flavin ring. To provide insight into the PAO substrate specificity and, amine oxidation mechanism, we have investigated the crystal structure of, maize PAO in the reduced state and in complex with three different, inhibitors, guazatine, 1,8-diaminooctane, and, N(1)-ethyl-N(11)-[(cycloheptyl)methyl]-4,8-diazaundecane (CHENSpm). In the, reduced state, the conformation of the isoalloxazine ring and the, surrounding residues is identical to that of the oxidized enzyme. Only, Lys300 moves away from the flavin to compensate for the change in cofactor, protonation occurring upon reduction. The structure of the PAO.inhibitor, complexes reveals an exact match between the inhibitors and the PAO, catalytic tunnel. Inhibitor binding does not involve any protein, conformational change. Such lock-and-key binding occurs also in the, complex with CHENSpm, which forms a covalent adduct with the flavin N5, atom. Comparison of the enzyme complexes hints at an "out-of-register", mechanism of inhibition, in which the inhibitor secondary amino groups are, not properly aligned with respect to the flavin to allow oxidation. Except, for the Glu62-Glu170 pair, no negatively charged residues are involved in, the recognition of substrate and inhibitor amino groups, which is in, contrast to other polyamine binding proteins. This feature may be, exploited in the design of drugs specifically targeting PAO.
About this StructureAbout this Structure
1H83 is a Single protein structure of sequence from Zea mays with NAG, FAD and DIA as ligands. Active as Polyamine oxidase, with EC number 1.5.3.11 Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.
ReferenceReference
Structural bases for inhibitor binding and catalysis in polyamine oxidase., Binda C, Angelini R, Federico R, Ascenzi P, Mattevi A, Biochemistry. 2001 Mar 6;40(9):2766-76. PMID:11258887
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