1ux9: Difference between revisions

Revision as of 11:48, 3 May 2008

MAPPING PROTEIN MATRIX CAVITIES IN HUMAN CYTOGLOBIN THROUGH XE ATOM BINDING: A CRYSTALLOGRAPHIC INVESTIGATION

OverviewOverview

Cytoglobin is the fourth recognized globin type, almost ubiquitously distributed in human tissues; its function is still poorly understood. Cytoglobin displays a core region of about 150 residues, structurally related to hemoglobin and myoglobin, and two extra segments, about 20 residues each, at the N- and C-termini. The core region hosts a large apolar cavity, held to provide a ligand diffusion pathway to/from the heme, and/or ligand temporary docking sites. Here we report the crystal structure (2.4A resolution, R-factor 19.1%) of a human cytoglobin mutant bearing the CysB2(38) --> Ser and CysE9(83) --> Ser substitutions (CYGB*), treated under pressurized xenon. Three Xe atoms bind to the heme distal site region of CYGB* mapping the protein matrix apolar cavity. Despite the conserved globin fold, the cavity found in CYGB* is structured differently from those recognized to play a functional role in myoglobin, neuroglobin, truncated hemoglobins, and Cerebratulus lacteus mini-hemoglobin.

About this StructureAbout this Structure

1UX9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Mapping protein matrix cavities in human cytoglobin through Xe atom binding., de Sanctis D, Dewilde S, Pesce A, Moens L, Ascenzi P, Hankeln T, Burmester T, Bolognesi M, Biochem Biophys Res Commun. 2004 Apr 16;316(4):1217-21. PMID:15044115 Page seeded by OCA on Sat May 3 11:48:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1ux9.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1ux9 |SIZE=350|CAPTION= <scene name='initialview01'>1ux9</scene>, resolution 2.4&Aring;
+The line below this paragraph, containing "STRUCTURE_1ux9", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Fc6+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FC6:HEXACYANOFERRATE(3-)'>FC6</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XE:XENON'>XE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ux9|  PDB=1ux9  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ux9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ux9 OCA], [http://www.ebi.ac.uk/pdbsum/1ux9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ux9 RCSB]</span>
-}}
 '''MAPPING PROTEIN MATRIX CAVITIES IN HUMAN CYTOGLOBIN THROUGH XE ATOM BINDING: A CRYSTALLOGRAPHIC INVESTIGATION'''
@@ Line 33: / Line 30: @@
 [[Category: Pesce, A.]]
 [[Category: Sanctis, D De.]]
-[[Category: heme]]
+[[Category: Heme]]
-[[Category: oxygen storage/transport]]
+[[Category: Oxygen storage/transport]]
-[[Category: transport]]
+[[Category: Transport]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:48:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:16:37 2008''