Endonuclease: Difference between revisions
Michal Harel (talk | contribs) No edit summary |
Michal Harel (talk | contribs) No edit summary |
||
| Line 118: | Line 118: | ||
**[[4mak]] – EcCas2<br /> | **[[4mak]] – EcCas2<br /> | ||
**[[4p6i]], [[4qdl]] – EcCas1 + EcCas2<br /> | **[[4p6i]], [[4qdl]] – EcCas1 + EcCas2<br /> | ||
**[[3s4l]] – MjCas3 – ''Methanocaldococcus jannaschii''<br /> | **[[3s4l]] – MjCas3 – ''Methanocaldococcus jannaschii''<br /> | ||
**[[4qqw]] – TfCas3 – ''Thermobifida fusca''<br /> | **[[4qqw]] – TfCas3 – ''Thermobifida fusca''<br /> | ||
| Line 128: | Line 127: | ||
**[[3i4h]] – Cas4 - ''Pyrobaculum calidifontis''<br /> | **[[3i4h]] – Cas4 - ''Pyrobaculum calidifontis''<br /> | ||
**[[4qr0]], [[4qr1]] - SpCas9 (Csn1) – ''Streptococcus pyogenes''<br /> | **[[4qr0]], [[4qr1]] - SpCas9 (Csn1) – ''Streptococcus pyogenes''<br /> | ||
**[[4ilr]], [[3zfv]] – SsCas6 <br /> | **[[4ilr]], [[3zfv]] – SsCas6 <br /> | ||
**[[3i4h]] – PfCas6 - ''Pyrococcus furiosus''<br /> | **[[3i4h]] – PfCas6 - ''Pyrococcus furiosus''<br /> | ||
**[[4dzd]] – EcCas6e<br /> | **[[4dzd]] – EcCas6e<br /> | ||
**[[4qr0]], [[4qr1]], [[4cmp]], [[4cmq]] - SpCas9 <br /> | **[[4qr0]], [[4qr1]], [[4cmp]], [[4cmq]] - SpCas9 <br /> | ||
**[[4ogc]], [[4oge]] – Cas9 II-C – ''Actinomyces naeslundii''<br /> | **[[4ogc]], [[4oge]] – Cas9 II-C – ''Actinomyces naeslundii''<br /> | ||
**[[3ung]], [[3ur3]], [[4doz]], [[4w8y]] – PfCas10 CMR2 subunit <br /> | **[[3ung]], [[3ur3]], [[4doz]], [[4w8y]] – PfCas10 CMR2 subunit <br /> | ||
**[[4h4k]] – PfCas10 CMR2+CMR3 subunits <br /> | **[[4h4k]] – PfCas10 CMR2+CMR3 subunits <br /> | ||
| Line 158: | Line 143: | ||
**[[4h7a]] - TtCasB (Cse2)<br /> | **[[4h7a]] - TtCasB (Cse2)<br /> | ||
**[[4tvx]] – EcCasA (Cse1) + CasE<br /> | **[[4tvx]] – EcCasA (Cse1) + CasE<br /> | ||
**[[3qhq]] - Csn2 – ''Streptococcus agalactiae''<br /> | **[[3qhq]] - Csn2 – ''Streptococcus agalactiae''<br /> | ||
**[[3toc]], [[3v7f]] – SpCsn2<br /> | **[[3toc]], [[3v7f]] – SpCsn2<br /> | ||
**[[3ps0]] – SsCsa2<br /> | **[[3ps0]] – SsCsa2<br /> | ||
*Cas ENN complex with RNA and DNA | |||
**[[5dlj]], [[5dqt]], [[5dqu]], [[5dqz]], [[5ds4]], [[5ds5]], [[5ds6]] – EcCas1 + EcCas2 + DNA<br /> | |||
**[[4ill]], [[4ilm]] – SsCas6 + RNA - ''Sulfolobus solfataricus''<br /> | |||
**[[5uz9]] – PaCsa5f + Cas7f + Cas8f + anti-crispr protein + RNA<br /> | |||
**[[3pkm]] – PfCas6 + RNA<br /> | |||
**[[2y8w]], [[2y8y]], [[3qrp]], [[3qrq]], [[2qrr]] – TtCas6e (Cse3) + RNA<br /> | |||
**[[2xli]], [[2xlj]], [[2xlk]], [[4al7]] – PaCas6f (Csy4) + RNA <br /> | |||
**[[4al5]], [[4al6]] - PaCas6f (Csy4) (mutant) + RNA <br /> | |||
**[[4zt0]] - SpCas9 + RNA <br /> | |||
**[[4zt9]] - SpCas9 (mutant) + RNA <br /> | |||
**[[5f9r]] - SpCas9 + RNA + DNA<br /> | |||
**[[4oo8]], [[4un3]], [[4un4]], [[4un5]], [[5fw1]], [[5fw2]], [[5fw3]], [[5fq5]], [[5b2r]], [[5b2s]], [[5b2t]] - SpCas9 (Csn1) (mutant) + RNA + DNA<br /> | |||
**[[5x2h]], [[5x2g]] – Cas9 + RNA + DNA – ''Campylobacter jejuni''<br /> | |||
**[[5czz]] - SaCas9 (Csn1) + RNA + DNA - ''Staphylococcus aureus''<br /> | |||
**[[5axw]] - SaCas9 (Csn1) (mutant) + RNA + DNA <br /> | |||
**[[5b2o]], [[5b2p]], [[5b2q]] - FtCas9 (mutant) + RNA + DNA – ''Francisella tularensis''<br /> | |||
**[[4u7u]] – EcCasA (Cse1) + CasB + CasC + CasD + CasE + RNA<br /> | |||
**[[4qyz]] – EcCasA (Cse1) + CasB + CasC + CasD + CasE + RNA + DNA<br /> | |||
**[[5wqe]] – AaCas C2C1 + RNA – ''Alicyclobacillus acidoterrestris''<br /> | **[[5wqe]] – AaCas C2C1 + RNA – ''Alicyclobacillus acidoterrestris''<br /> | ||
**[[5u34]] – AaCas C2C1 (mutant) + RNA <br /> | **[[5u34]] – AaCas C2C1 (mutant) + RNA <br /> | ||
Revision as of 13:28, 30 April 2017
FunctionEndonuclease (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN[1]. The Cas ENN proteins are part of CRISPR/Cas prokaryotic immune system which confers protection from foreign genetic elements like viruses. The CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool[2]. For more details see
Intron-encoded ENN or homing ENN are encoded by genes with mobile, self-splicing introns. They promote the movement of DNA sequences from one chromosome location to another[3]. See also
RelevanceSickle cell anemia is caused by mutation in the recognition site of MstII ENN. DiseaseMutation in UV-specific ENN causes Xeroderma pigmentosa. Mutations in tRNA-splicing ENN cause pontocerebellar hypoplasia. Structural highlights
(PDB code 1rva). |
| ||||||||||
3D structures of endonuclease3D structures of endonuclease
Updated on 30-April-2017
ReferencesReferences
- ↑ Nishino T, Morikawa K. Structure and function of nucleases in DNA repair: shape, grip and blade of the DNA scissors. Oncogene. 2002 Dec 16;21(58):9022-32. PMID:12483517 doi:http://dx.doi.org/10.1038/sj.onc.1206135
- ↑ Horvath P, Barrangou R. CRISPR/Cas, the immune system of bacteria and archaea. Science. 2010 Jan 8;327(5962):167-70. doi: 10.1126/science.1179555. PMID:20056882 doi:http://dx.doi.org/10.1126/science.1179555
- ↑ Flick KE, Jurica MS, Monnat RJ Jr, Stoddard BL. DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI. Nature. 1998 Jul 2;394(6688):96-101. PMID:9665136 doi:10.1038/27952