1ulf: Difference between revisions

Revision as of 11:23, 3 May 2008

CGL2 in complex with Blood Group A tetrasaccharide

OverviewOverview

Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted oligosaccharides of the mammalian blood group A/B type in an extended binding cleft. Kinetic studies on wild-type and mutant CGL2 proteins demonstrate that the tetrameric organization is essential for functionality. The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity.

About this StructureAbout this Structure

1ULF is a Single protein structure of sequence from Coprinopsis cinerea. Full crystallographic information is available from OCA.

ReferenceReference

Structure and functional analysis of the fungal galectin CGL2., Walser PJ, Haebel PW, Kunzler M, Sargent D, Kues U, Aebi M, Ban N, Structure. 2004 Apr;12(4):689-702. PMID:15062091 Page seeded by OCA on Sat May 3 11:23:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ulf.jpg|left|200px]]
- {{Structure
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-|GENE= cgl2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5346 Coprinopsis cinerea])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1sla|1SLA]], [[1qmj|1QMJ]], [[1gan|1GAN]], [[1c1f|1C1F]], [[1bkz|1BKZ]], [[1a3k|1A3K]], [[1lcl|1LCL]], [[1is5|1IS5]], [[1ul9|1UL9]], [[1ulc|1ULC]], [[1uld|1ULD]], [[1ule|1ULE]], [[1ulg|1ULG]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ulf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ulf OCA], [http://www.ebi.ac.uk/pdbsum/1ulf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ulf RCSB]</span>
-}}
 '''CGL2 in complex with Blood Group A tetrasaccharide'''
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 [[Category: Kues, U.]]
 [[Category: Walser, P J.]]
-[[Category: beta-galactoside binding lectin]]
+[[Category: Beta-galactoside binding lectin]]
-[[Category: galectin]]
+[[Category: Galectin]]
-[[Category: lectin]]
+[[Category: Lectin]]
-[[Category: sugar binding]]
+[[Category: Sugar binding]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:23:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:11:53 2008''