1ue7: Difference between revisions

Revision as of 11:05, 3 May 2008

Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis

OverviewOverview

Single-stranded DNA-binding protein (SSB) is an essential protein necessary for the functioning of the DNA replication, repair and recombination machineries. Here we report the structure of the DNA-binding domain of Mycobacterium tuberculosis SSB (MtuSSB) in four different crystals distributed in two forms. The structure of one of the forms was solved by a combination of isomorphous replacement and anomalous scattering. This structure was used to determine the structure of the other form by molecular replacement. The polypeptide chain in the structure exhibits the oligonucleotide binding fold. The globular core of the molecule in different subunits in the two forms and those in Escherichia coli SSB (EcoSSB) and human mitochondrial SSB (HMtSSB) have similar structure, although the three loops exhibit considerable structural variation. However, the tetrameric MtuSSB has an as yet unobserved quaternary association. This quaternary structure with a unique dimeric interface lends the oligomeric protein greater stability, which may be of significance to the functioning of the protein under conditions of stress. Also, as a result of the variation in the quaternary structure the path adopted by the DNA to wrap around MtuSSB is expected to be different from that of EcoSSB.

About this StructureAbout this Structure

1UE7 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M, J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:12888346 Page seeded by OCA on Sat May 3 11:05:56 2008

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OCA

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 [[Image:1ue7.gif|left|200px]]
- {{Structure
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-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04496 SSB_OBF]</span>
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-|RELATEDENTRY=[[1ue1|1UE1]], [[1ue5|1UE5]], [[1ue6|1UE6]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ue7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ue7 OCA], [http://www.ebi.ac.uk/pdbsum/1ue7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ue7 RCSB]</span>
-}}
 '''Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis'''
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 [[Category: Venkatesh, J.]]
 [[Category: Vijayan, M.]]
-[[Category: dna-binding protein]]
+[[Category: Dna-binding protein]]
-[[Category: oligonucleotide binding fold]]
+[[Category: Oligonucleotide binding fold]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: tb structural genomics consortium]]
+[[Category: Tb structural genomics consortium]]
-[[Category: tbsgc]]
+[[Category: Tbsgc]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:05:56 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:09:05 2008''