1u7c: Difference between revisions

Revision as of 10:50, 3 May 2008

Crystal Structure of AmtB from E.Coli with Methyl Ammonium.

OverviewOverview

The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.

About this StructureAbout this Structure

1U7C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A., Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM, Science. 2004 Sep 10;305(5690):1587-94. PMID:15361618 Page seeded by OCA on Sat May 3 10:50:54 2008

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OCA

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 [[Image:1u7c.jpg|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1u7c", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NME:METHYLAMINE'>NME</scene>
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+or leave the SCENE parameter empty for the default display.
-|GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1u7c|  PDB=1u7c  |  SCENE=  }}
-|RELATEDENTRY=[[1u77|1U77]], [[1u7g|1U7G]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u7c OCA], [http://www.ebi.ac.uk/pdbsum/1u7c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u7c RCSB]</span>
-}}
 '''Crystal Structure of AmtB from E.Coli with Methyl Ammonium.'''
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 [[Category: Robles-Colmenares, Y.]]
 [[Category: Stroud, R M.]]
-[[Category: ammonia channel]]
+[[Category: Ammonia channel]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
-[[Category: methy ammonium]]
+[[Category: Methy ammonium]]
-[[Category: right handed helical bundle]]
+[[Category: Right handed helical bundle]]
-[[Category: transmembrane helice]]
+[[Category: Transmembrane helice]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:50:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:06:18 2008''